Eicosapentaenoic Acid, a n-3 Polyunsaturated Fatty Acid Differentially Modulates TNF- α, IL-1α, IL-6 and PGE2 Expression in UVB-Irradiated Human Keratinocytes

Slides:

Advertisements

Similar presentations

Glucocorticoids Augment the Chemically Induced Production and Gene Expression of Interleukin-1α through NF-κB and AP-1 Activation in Murine Epidermal.

Advertisements

UVB Increases Urokinase-Type Plasminogen Activator Receptor (uPAR) Expression1 Christoph Marschall, Toshiko Nobutoh, Evelyn Braungart, Kathrin Douwes,

The Suppressor of Cytokine Signaling-3 Is Upregulated in Impaired Skin Repair: Implications for Keratinocyte Proliferation Itamar Goren, Andreas Linke,

Expression of Human Macrophage Metalloelastase (MMP-12) by Tumor Cells in Skin Cancer Erja Kerkelä, Risto Ala-aho, Leila Jeskanen, Oona Rechardt, Reidar.

Sphingosylphosphorylcholine is a Potent Inducer of Intercellular Adhesion Molecule-1 Expression in Human Keratinocytes Genji Imokawa, Yutaka Takagi,

A Toll-Like Receptor 7, 8, and 9 Antagonist Inhibits Th1 and Th17 Responses and Inflammasome Activation in a Model of IL-23-Induced Psoriasis Weiwen.

Role of Monocyte Chemoattractant Protein-1 and its Receptor,CCR-2, in the Pathogenesis of Bleomycin-Induced Scleroderma Toshiyuki Yamamoto, Kiyoshi Nishioka

Modulation of Microphthalmia-associated Transcription Factor Gene Expression Alters Skin Pigmentation C.B. Lin, L. Babiarz, F. Liebel, M. Kizoulis, G.J.

Activation of Tissue Inhibitor of Metalloproteinases-3 (TIMP-3) mRNA Expression in Scleroderma Skin Fibroblasts Laura Mattila, Kristiina Airola, Matti.

Keratinocyte-Releasable Stratifin Functions as a Potent Collagenase-Stimulating Factor in Fibroblasts Aziz Ghahary, Feridoun Karimi-Busheri, Yvonne Marcoux,

Membrane Type 1 Matrix Metalloproteinase Regulates Cellular Invasiveness and Survival in Cutaneous Epidermal Cells Usha Nagavarapu, Kenneth Relloma,

IGF-II-Mediated COX-2 Gene Expression in Human Keratinocytes Through Extracellular Signal-Regulated Kinase Pathway Hye Jung Kim, Tae-Yoon Kim Journal.

Iontophoresis and Sonophoresis Stimulate Epidermal Cytokine Expression at Energies That Do Not Provoke a Barrier Abnormality: Lamellar Body Secretion.

Suppression of Vitamin D Receptor and Induction of Retinoid X Receptor α Expression During Squamous Differentiation of Cultured Keratinocytes Siegfried.

Green Tea Polyphenols Prevent Ultraviolet Light-Induced Oxidative Damage and Matrix Metalloproteinases Expression in Mouse Skin Praveen K. Vayalil, Anshu.

Ultraviolet Light and Interleukin-10 Modulate Expression of Cytokines by Transformed Human Dermal Microvascular Endothelial Cells (HMEC-1) Thomas Scholzen,

Characterization of Group X Phospholipase A2 as the Major Enzyme Secreted by Human Keratinocytes and its Regulation by the Phorbol Ester TPA Gérard Lambeau,

Anti-Inflammatory Activity of Sertaconazole Nitrate Is Mediated via Activation of a p38– COX-2–PGE2 Pathway Runa Sur, Jeffrey M. Babad, Michelle Garay,

Role of p38 MAPK in Transforming Growth Factor β Stimulation of Collagen Production by Scleroderma and Healthy Dermal Fibroblasts Madoka Sato, Daniel.

Davina A. Lewis, Simone F. Hengeltraub, Feng C. Gao, Megan A

Sema4D, the Ligand for Plexin B1, Suppresses c-Met Activation and Migration and Promotes Melanocyte Survival and Growth Joanne Soong, Yulin Chen, Elina.

Supriya Kapas, Paula M. Farthing Journal of Investigative Dermatology

AKT Status Controls Susceptibility of Malignant Keratinocytes to the Early-Activated and UVB-Induced Apoptotic Pathway David Decraene, An Van Laethem,

UV Induces p21WAF1/CIP1 Protein in Keratinocytes Without p53

Functional Characterization of IL-17F as a Selective Neutrophil Attractant in Psoriasis Hideaki Watanabe, Mio Kawaguchi, Sawa Fujishima, Miyoko Ogura,

Suppressor of Cytokine Signaling 1/JAB and Suppressor of Cytokine Signaling 3/Cytokine-Inducible SH2 Containing Protein 3 Negatively Regulate the Signal.

All-Trans-Retinoic Acid Induces Interleukin-8 via the Nuclear Factor-κB and p38 Mitogen-Activated Protein Kinase Pathways in Normal Human Keratinocytes

Kellie J. White, Vincent J. Maffei, Marvin Newton-West, Robert A

17β-estradiol Inhibits the Production of RANTES in Human Keratinocytes

Advanced Inhibition of Undesired Human Hair Growth by PPARγ Modulation? Yuval Ramot, Arianna Mastrofrancesco, Erika Herczeg-Lisztes, Tamás Bíró, Mauro.

Different Susceptibility of Malignant versus Nonmalignant Human T Cells Toward Ultraviolet A-1 Radiation-Induced Apoptosis Ritsuko Yamauchi, Akimichi.

Volume 61, Issue 6, Pages (June 2002)

UVB and Proinflammatory Cytokines Synergistically Activate TNF-α Production in Keratinocytes through Enhanced Gene Transcription Muhammad M. Bashir,

Human Keratinocytes Respond to Osmotic Stress by p38 Map Kinase Regulated Induction of HSP70 and HSP27 M. Garmyn, A. Pupe Journal of Investigative Dermatology

SIRT1, a Class III Histone Deacetylase, Regulates LPS-Induced Inflammation in Human Keratinocytes and Mediates the Anti-Inflammatory Effects of Hinokitiol

Dermatopontin Expression is Decreased in Hypertrophic Scar and Systemic Sclerosis Skin Fibroblasts and is Regulated by Transforming Growth Factor-β1,

Hyaluronan Synthase 3 Regulates Hyaluronan Synthesis in Cultured Human Keratinocytes Tetsuya Sayo, Yoshinori Sugiyama, Yoshito Takahashi, Naoko Ozawa,

Liang Deng, Wanhong Ding, Richard D. Granstein

Connective Tissue Growth Factor: Expression in Human Skin In Vivo and Inhibition by Ultraviolet Irradiation Taihao Quan, Tianyuan He, Sewon Kang, John.

Syed M. Meeran, Thejass Punathil, Santosh K. Katiyar

Regulation of Guanylate-Binding Protein Expression in Interferon-γ-Treated Human Epidermal Keratinocytes and Squamous Cell Carcinoma Cells Nicholas A.

Karen Hanley, Yan Jiang, Shan Shan He, Mark Friedman, Peter M

The Effect of Thioredoxin on the Expression of Proopiomelanocortin-Derived Peptides, the Melanocortin 1 Receptor and Cell Survival of Normal Human Keratinocytes

Research Snippets Journal of Investigative Dermatology

The Effect of LXR Activators on AP-1 Proteins in Keratinocytes

Corticotropin-Releasing Hormone (CRH) Downregulates Interleukin-18 Expression in Human HaCaT Keratinocytes by Activation of p38 Mitogen-Activated Protein.

A p38MAPK/HIF-1 Pathway Initiated by UVB Irradiation Is Required to Induce Noxa and Apoptosis of Human Keratinocytes Kris Nys, An Van Laethem, Carine.

Post-Transcriptional Regulation of UV Induced TNF-α Expression

1α,25-Dihydroxyvitamin D3 Stimulates Activator Protein 1 DNA-Binding Activity by a Phosphatidylinositol 3-Kinase/Ras/MEK/Extracellular Signal Regulated.

Lawrence M. Pfeffer, Andrzej T. Slominski

Keratinocytes Inhibit Expression of Connective Tissue Growth Factor in Fibroblasts In Vitro by an Interleukin-1α-Dependent Mechanism Daniel Nowinski,

Blazej Zbytek, Andrzej T. Slominski

Journal of Investigative Dermatology

Transient Receptor Potential Vanilloid-1 Mediates Heat-Shock-Induced Matrix Metalloproteinase-1 Expression in Human Epidermal Keratinocytes Wen H. Li,

Topical Application of a Novel, Hydrophilic γ-Tocopherol Derivative Reduces Photo- Inflammation in Mice Skin Emiko Yoshida, Tatsuya Watanabe, Jiro Takata,

Extracellular ATP Has Stimulatory Effects on the Expression and Release of IL-6 Via Purinergic Receptors in Normal Human Epidermal Keratinocytes Kaori.

Naoko Kanda, Shinichi Watanabe Journal of Investigative Dermatology

Matthias Lüftl, Martin Röcken, Gerd Plewig, Klaus Degitz

All-Trans Retinoic Acid Antagonizes UV-Induced VEGF Production and Angiogenesis via the Inhibition of ERK Activation in Human Skin Keratinocytes Mi-Sun.

Involvement of PPARγ in Oxidative Stress-Mediated Prostaglandin E2 Production in SZ95 Human Sebaceous Gland Cells Qiwei Zhang, Holger Seltmann, Christos.

Journal of Investigative Dermatology

Ultraviolet B Radiation Upregulates the Production of Macrophage Migration Inhibitory Factor (MIF) in Human Epidermal Keratinocytes Tadamichi Shimizu,

Cis-Urocanic Acid Stimulates Primary Human Keratinocytes Independently of Serotonin or Platelet-Activating Factor Receptors Kazuyo Kaneko, Jeffrey B.

IL-12 Completely Blocks Ultraviolet-Induced Secretion of Tumor Necrosis Factor α from Cultured Skin Fibroblasts and Keratinocytes Victoria P. Werth,

Modulation of IL-10, IL-12, and IFN-γ in the Epidermis of Hairless Mice by UVA (320– 400 nm) and UVB (280–320 nm) Radiation Jie Shen, Shisan Bao, Vivienne.

Hyun Jeong Park, Hee Jung Kim, Jun Young Lee, Baik Kee Cho, Richard L

Runa Sur, Peter A. Lyte, Michael D. Southall

Regulation of IL-13 Receptors in Human Keratinocytes

The Activity of Caspase-1 Is Increased in Lesional Psoriatic Epidermis

Clusterin Gene Expression Mediates Resistance to Apoptotic Cell Death Induced by Heat Shock and Oxidative Stress Isabelle Viard, Philippe Wehrli, Lan.

Presentation transcript:

Eicosapentaenoic Acid, a n-3 Polyunsaturated Fatty Acid Differentially Modulates TNF- α, IL-1α, IL-6 and PGE2 Expression in UVB-Irradiated Human Keratinocytes Annemie Pupe, Petra De Haes, Lesley Rhodes, Marjan Garmyn Journal of Investigative Dermatology Volume 118, Issue 4, Pages 692-698 (April 2002) DOI: 10.1046/j.1523-1747.2002.01615.x Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 EPA treatment results in higher TNF-α and IL-1α mRNA expression in sham- and UVB-irradiated NHK. (a) NHK were pretreated with vehicle or EPA and UVB-irradiated as described in Materials and Methods. Total RNA was isolated at different time points postirradiation. Northern blotting was performed with hybridization of 32P-labeled probes for TNF-α, IL-1α, and GAPDH. One representative northern blot is shown. (b) Densitometric quantification of TNF-α and IL-1α mRNA levels normalized for GAPDH mRNA levels. Data are relative to control (vehicle; -UVB) which is arbitrarily set at 1. Journal of Investigative Dermatology 2002 118, 692-698DOI: (10.1046/j.1523-1747.2002.01615.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 EPA treatment results in higher TNF-α secretion in sham- and UVB-irradiated NHK. Medium was collected at various time points postirradiation from NHK treated in the same way as depicted in Figure 1. This medium was then analyzed for TNF-α secretion by means of ELISA. Results are shown as mean ± SD (n = 2) of one representative experiment. Journal of Investigative Dermatology 2002 118, 692-698DOI: (10.1046/j.1523-1747.2002.01615.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 EPA treatment results in higher IL-1α secretion in sham- and UVB-irradiated NHK. Medium was collected in the same way as depicted in Figure 2. This medium was then analyzed for IL-1α secretion using ELISA. Results are shown as mean ± SD (n = 2) of one representative experiment. Journal of Investigative Dermatology 2002 118, 692-698DOI: (10.1046/j.1523-1747.2002.01615.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 EPA treatment results in inhibition of UVB-induced IL-6 secretion in NHK. Medium was collected in the same way as depicted in Figure 2. This medium was diluted 10 times in medium and then analyzed for IL-6 secretion using ELISA, according to manufacturer's protocol. Results are shown as mean ± SD (n = 2) of one representative experiment. Journal of Investigative Dermatology 2002 118, 692-698DOI: (10.1046/j.1523-1747.2002.01615.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 EPA treatment results in increased lipid peroxidation in UVB-irradiated NHK. NHK were pretreated for 24 h with vehicle or EPA and then UVB-irradiated as described in Materials and Methods. Cells were washed and collected in PBS and then resuspended in 100 µl MilliQ water 6 h later. Lipid peroxidation products (malondialdehyde; MDA) were measured as described in Materials and Methods. MDA levels in cell lysates are expressed as pmol per µg protein. Journal of Investigative Dermatology 2002 118, 692-698DOI: (10.1046/j.1523-1747.2002.01615.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 EPA treatment reduces UVB-induced PGE2 levels in NHK. Medium was collected in the same way as depicted in Figure 2. This medium was diluted 10 times in medium and then analyzed for PGE2 secretion using competitive ELISA. Results are shown as mean ± SD (n = 2) of one representative experiment. Journal of Investigative Dermatology 2002 118, 692-698DOI: (10.1046/j.1523-1747.2002.01615.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 7 Effects on TNF-α and IL-1α mRNA expression are specific for EPA. (a) NHK were treated with vehicle, OA, EPA, or LA for 24 h. Total RNA was extracted at 5 h postirradiation. Northern blotting was performed as described in Figure 1. One representative northern blot is shown. (b) Densitometric quantification of TNF-α and IL-1α mRNA levels normalized for GAPDH mRNA levels. Data are relative to control (vehicle; -UVB) which is arbitrarily set at 1. Journal of Investigative Dermatology 2002 118, 692-698DOI: (10.1046/j.1523-1747.2002.01615.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions