Volume 139, Issue 3, Pages 394-400 (December 2015) Suboptimal cytoreduction in ovarian carcinoma is associated with molecular pathways characteristic of increased stromal activation  Zhenqiu Liu, Jessica A. Beach, Hasmik Agadjanian, Dongyu Jia, Paul-Joseph Aspuria, Beth Y. Karlan, Sandra Orsulic  Gynecologic Oncology  Volume 139, Issue 3, Pages 394-400 (December 2015) DOI: 10.1016/j.ygyno.2015.08.026 Copyright © 2015 Terms and Conditions

Fig. 1 Identification and validation of the suboptimal cytoreduction associated network (SCAN). (A) Statistical analysis workflow chart. (B) Selected biomarkers with both differentially expressed genes and differential networks. (C) External validation of the network genes in the validation dataset (GSE9891). The top four genes with the lowest P values are highlighted in red. (D) Predicted Area Under the ROC Curve (AUC) for the SCAN genes in the validation dataset (GSE9891). Gynecologic Oncology 2015 139, 394-400DOI: (10.1016/j.ygyno.2015.08.026) Copyright © 2015 Terms and Conditions

Fig. 2 The SCAN genes are highly enriched in the C1/mesenchymal molecular subtypes of EOC. Expression levels of the SCAN genes in (A) differentiated, immunoreactive, mesenchymal, and proliferative molecular subtypes in the TCGA dataset and (B) C1–C5 molecular subtypes in the Tothill dataset. Gynecologic Oncology 2015 139, 394-400DOI: (10.1016/j.ygyno.2015.08.026) Copyright © 2015 Terms and Conditions

Fig. 3 EOCs characterized by lymphatic and venous invasion express higher levels of the SCAN genes. Relative expression levels of the SCAN genes in patient samples based on the presence or absence of (A) lymphatic invasion and (B) venous invasion. The number of samples in each category is indicated in parentheses. Gynecologic Oncology 2015 139, 394-400DOI: (10.1016/j.ygyno.2015.08.026) Copyright © 2015 Terms and Conditions

Fig. 4 The SCAN genes are enriched in the cancer-associated stroma. (A) Relative expression levels of the SCAN genes in microdissected stromal and epithelial components in the normal ovary and in ovarian cancer. (B) Relative expression levels of the overall stromal marker VIM, reactive stroma marker ACTA2, and the SCAN genes in patient-matched omental metastases and primary tumors. The number of samples in each category is indicated in parentheses. (C) Representative images of sections of a recurrent serous ovarian tumor show COL11A1 expression in a subset of α-SMA-positive fibroblasts. Expression of COL11A1 was determined by in situ hybridization (ISH) with a COL11A1-specific probe. The presence of tumor stroma was determined by immunohistochemical (IHC) staining with α-SMA. The distribution of collagen and smooth muscle connective tissue was determined by Masson's trichrome staining. Gynecologic Oncology 2015 139, 394-400DOI: (10.1016/j.ygyno.2015.08.026) Copyright © 2015 Terms and Conditions