QRT–PCR analysis of tissue‐specific candidate gene expression in response to reduced IIS qRT–PCR analysis of tissue‐specific candidate gene expression.

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qRT–PCR analysis of tissue‐specific candidate gene expression in response to reduced IIS qRT–PCR analysis of tissue‐specific candidate gene expression in response to reduced IIS A–CqRT–PCR analysis shows gene expression levels of CG2014, CG6463, CoIV, CG4169, CG3731 in the fat body (A), or of Rpn6, Rpt6R, Rpn11 and Pros‐alpha3 in the gut (B, C) of control (wDah), dfoxo mutant (dfoxo94), mNSC‐ablated (InsP3‐Gal4/UAS‐rpr) and mNSC‐ablated flies in the absence of dfoxo (InsP3‐Gal4/UAS‐rpr, dfoxo94). Relative expression levels are normalized to either RPL32 (A) or Actin5c (B, C). Significance established by two‐way ANOVA and post hoc pairwise tests (n = 3, or otherwise shown). Bars indicate mean SEM (*P < 0.05; **P < 0.01; ***P < 0.001). Luke S Tain et al. Mol Syst Biol 2017;13:939 © as stated in the article, figure or figure legend