Formation of functional heterologous complexes using subunits from the picromycin, erythromycin and oleandomycin polyketide synthases Li Tang, Hong Fu,

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Presentation transcript:

Formation of functional heterologous complexes using subunits from the picromycin, erythromycin and oleandomycin polyketide synthases Li Tang, Hong Fu, Robert McDaniel Chemistry & Biology Volume 7, Issue 2, Pages 77-84 (February 2000) DOI: 10.1016/S1074-5521(00)00073-9

Figure 1 Modular arrangement of the related PKSs that produce (a) 6-deoxyerythronolide B (DEBS), (b) 8,8a-deoxyoleandolide (OlePKS), and (c) narbonolide (PikPKS). Each PKS consists of six modules, each of which catalyzes a round of condensation and subsequent β-keto modification on the growing polyketide chain. The colored modules highlight the differences in activities between each PKS system and the polyketide produced ([23,24]; R.M., unpublished observations). KS, ketosynthase; AT, acyltransferase; ACP, acyl carrier protein; KR, ketoreductase; DH, dehydratase; ER, enoylreductase; TE, thioesterase; KSQ, putative decarboxylation domain [32,33]. Chemistry & Biology 2000 7, 77-84DOI: (10.1016/S1074-5521(00)00073-9)

Figure 2 Polyketides produced by complementation with heterologous PKS subunits from PikPKS (purple), DEBS (red), and OlePKS (orange). Chemistry & Biology 2000 7, 77-84DOI: (10.1016/S1074-5521(00)00073-9)

Figure 3 Combinatorial library production by complementation with genetically engineered PKS subunits from PikPKS (purple), DEBS (red) and domains from RAPS (green). Chemistry & Biology 2000 7, 77-84DOI: (10.1016/S1074-5521(00)00073-9)

Figure 4 Engineered PKS fusions between DEBS (red) and PikPKS (purple). NP, no product. Chemistry & Biology 2000 7, 77-84DOI: (10.1016/S1074-5521(00)00073-9)