M. Sabaté, E. Moreno, T. Pérez, A. Andreu, G. Prats 

Slides:



Advertisements
Similar presentations
Models for the organisation of hospital infection control and prevention programmes B. Gordts Clinical Microbiology and Infection Volume 11, Pages
Advertisements

C.-S. Lee, J.-H. Lee  Clinical Microbiology and Infection 
P.-Y. Lévy  Clinical Microbiology and Infection 
R. Dumke, H. von Baum, P.C. Lück, E. Jacobs 
A. Bergman, D. Heimer, N. Kondori, H. Enroth 
L. Boyanova  Clinical Microbiology and Infection 
Women with symptoms of a urinary tract infection but a negative urine culture: PCR- based quantification of Escherichia coli suggests infection in most.
Rapid and sensitive diagnosis of Toxoplasma gondii infections by PCR
Effect of comprehensive validation of the template isolation procedure on the reliability of bacteraemia detection by a 16S rRNA gene PCR  A. Heininger,
Detection of fungal DNA in lysis–centrifugation blood culture for the diagnosis of invasive candidiasis in neonatal patients  L. Trovato, P. Betta, M.G.
Gut bacterial microbiota and obesity
Activity of quinupristin–dalfopristin in invasive isolates of Streptococcus pneumoniae from Italy  A. Pantosti, F. D'Ambrosio, E. Bordi, A. Scotto d'Abusco,
M. Cogliati, R. D'Amicis, A.M. Tortorano 
X. Kong, T. Xiao, J. Lin, Y. Wang, H.-D. Chen 
C.-S. Lee, J.-H. Lee  Clinical Microbiology and Infection 
A comparison of three real-time PCR assays for the confirmation of Neisseria gonorrhoeae following detection of N. gonorrhoeae using Roche COBAS AMPLICOR 
G. Zhao, H. Zhai, Q. Yuan, S. Sun, T. Liu, L. Xie 
Prevalence of diarrheagenic Escherichia coli strains detected by PCR in patients with travelers' diarrhea  M. Vargas, J. Gascón, F. Gallardo, M. T. Jimenez.
Evidence of transmission of verocytotoxin-producing Escherichia coli O111 from a cattle stable to a child  E. Møller Nielsen, C. Jensen, D. Lau Baggesen 
Gentamicin susceptibility in Escherichia coli related to the genetic background: problems with breakpoints  L. Jakobsen, D. Sandvang, V.F. Jensen, A.M.
Integron-mediated antibiotic multiresistance in Acinetobacter baumannii clinical isolates from Spain  J. Ruiz, M.M. Navia, C. Casals, J.M. Sierra, M.T.
Molecular and phenotypic profiling of sorbitol-fermenting Escherichia coli O157:H− human isolates from Finland  M. Eklund, M. Bielaszewska, U.-M. Nakari,
A PCR-based method to differentiate between Acinetobacter baumannii and Acinetobacter genomic species 13TU  P.G. Higgins, H. Wisplinghoff, O. Krut, H.
P. Nordmann, L. Poirel  Clinical Microbiology and Infection 
Neonatal listeriosis in Algeria: the first two cases
New multiplex PCR method for the detection of Clostridium difficile toxin A (tcdA) and toxin B (tcdB) and the binary toxin (cdtA/cdtB) genes applied to.
Non-antibiotic strategies for sepsis
Assessment of the cag pathogenicity island status of Helicobacter pylori infections with serology and PCR  M. Kivi, Y. Tindberg, C. Bengtsson, L. Engstrand,
Women with symptoms of a urinary tract infection but a negative urine culture: PCR- based quantification of Escherichia coli suggests infection in most.
Clinical Microbiology and Infection
Surveillance of Legionnaires’ disease in Austria
A nested polymerase chain reaction for detection of Legionella pneumophila in clinical specimens  Sverker Bernander, Hanna-Stina Hanson, Bo Johansson,
Odile Launay, Jean-Claude Nguyen Van, Annie Buu-Hoï, J.F. Acar 
Effect of comprehensive validation of the template isolation procedure on the reliability of bacteraemia detection by a 16S rRNA gene PCR  A. Heininger,
Vector control: a cornerstone in the malaria elimination campaign
Distribution of rpoB mutations among multidrug-resistant Mycobacterium tuberculosis (MDRTB) strains from Thailand and development of a rapid method for.
Breast milk causing neonatal sepsis and death
A comparison of three real-time PCR assays for the confirmation of Neisseria gonorrhoeae following detection of N. gonorrhoeae using Roche COBAS AMPLICOR 
A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I–V  K. Boye, M.D. Bartels, I.S. Andersen, J.A. Møller,
Multiplex molecular testing for management of infectious gastroenteritis in a hospital setting: a comparative diagnostic and clinical utility study  E.
Prevalence of the sat, set and sen genes among diverse serotypes of Shigella flexneri strains isolated from patients with acute diarrhoea  S.K. Niyogi,
Antibiotic resistance patterns of Escherichia coli isolates from different aquatic environmental sources in Leon, Nicaragua  E. Amaya, D. Reyes, M. Paniagua,
Rapid identification of uropathogenic Escherichia coli of the O25:H4-ST131 clonal lineage using the Diversi-Lab repetitive sequence-based PCR system 
Elements of design: the knowledge on which we build
Gerda T. Noordhoek, Sitha A. Scheltinga, Paul Caesar, Leo M
A method for fast and simple detection of major diarrhoeagenic Escherichia coli in the routine diagnostic laboratory  S. Persson, K.E.P. Olsen, F. Scheutz,
Metagenomics and probiotics
Prevalence of human papilloma virus type 5 DNA in lesional and non-lesional skin scales of Italian plaque-type psoriatic patients: association with disease.
L. Vogel, E. van Oorschot, H.M.E. Maas, B. Minderhoud, L. Dijkshoorn 
R. Alonso, C. Muñoz, T. Peláez, E. Cercenado, M. Rodríguez-Creixems, E
T.M. File  Clinical Microbiology and Infection 
Phylogenetic group B2 Escherichia coli strains from the bowel microbiota of Pakistani infants carry few virulence genes and lack the capacity for long-term.
Update on antifungal resistance in Aspergillus and Candida
Harmonised monitoring of antimicrobial resistance in Salmonella and Campylobacter isolates from food animals in the European Union  S. Bronzwaer  Clinical.
A. Gelosia, L. Baldassarri, M. Deighton, T. van Nguyen 
Pandemic lineages of extraintestinal pathogenic Escherichia coli
A.P. Underwood, J. Green  Clinical Microbiology and Infection 
C. Gagliotti, L. Nobilio, M.L. Moro 
Prevalence and diversity of extended-spectrum ß-lactamases in faecal Escherichia coli isolates from healthy humans in Spain  L. Vinué, Y. Sáenz, S. Martínez,
Statin use and clinical outcomes among pneumonia patients
I. Bitar, A. Piazza  Clinical Microbiology and Infection 
The atypical pneumonias: clinical diagnosis and importance
Modelling during an emergency: the 2009 H1N1 influenza pandemic
J.L. Balcázar  Clinical Microbiology and Infection 
G.C. Schito  Clinical Microbiology and Infection 
Typing of Clostridium difficile
Impact of antibiotic restrictions: the patient's perspective
High prevalence of mutators among Enterobacter cloacae nosocomial isolates and their association with antimicrobial resistance and repetitive detection 
CMI readers' survey Clinical Microbiology and Infection
The future of diagnostic bacteriology
Presentation transcript:

Pathogenicity island markers in commensal and uropathogenic Escherichia coli isolates  M. Sabaté, E. Moreno, T. Pérez, A. Andreu, G. Prats  Clinical Microbiology and Infection  Volume 12, Issue 9, Pages 880-886 (September 2006) DOI: 10.1111/j.1469-0691.2006.01461.x Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 (A) Examples of multiplex PCR for detecting PAI markers IICFT073, III536, and IV536. Lanes: 1, Molecular Weight Marker VIII (Roche Diagnostics); 2 - 5, E. coli isolates; 6 and 7, positive control E. coli J96 and E. coli 536, respectively; and 8, negative control (reaction mix without added template DNA). (B) Examples of multiplex PCR for detecting PAI markers I536, II536, IJ96, IIJ96, and ICFT073. Lanes: 1, Molecular Weight Marker III (Roche Diagnostics); 2 - 5, E. coli isolates; 6, positive control E. coli J96; and 7, negative control (reaction mix without added template DNA). Clinical Microbiology and Infection 2006 12, 880-886DOI: (10.1111/j.1469-0691.2006.01461.x) Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Specific pathogenicity island (PAI) combinations among the 84 Escherichia coli isolates (commensal and uropathogenic) carrying more than one PAI. Clinical Microbiology and Infection 2006 12, 880-886DOI: (10.1111/j.1469-0691.2006.01461.x) Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions