Volume 7, Pages (September 2018)

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Volume 7, Pages 170-179 (September 2018) Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow Assay Chao Zhang, Xiaonan Liu, Yao Yao, Kewu Liu, Wenli Hui, Juanli Zhu, Yaling Dou, Kai Hua, Mingli Peng, Zuankai Wang, Alphonsus J.M. Vermorken, Yali Cui iScience Volume 7, Pages 170-179 (September 2018) DOI: 10.1016/j.isci.2018.09.005 Copyright © 2018 The Author(s) Terms and Conditions

iScience 2018 7, 170-179DOI: (10.1016/j.isci.2018.09.005) Copyright © 2018 The Author(s) Terms and Conditions

Figure 1 Schematic Diagram of the Direct PCR-MLFA System (A) Sample collection and treatment with NaOH. (B) Target amplification using specifically designed primers (corresponding to M and WT). (C) Signal readout: the composition and working principle of the magnetic lateral flow assay. When the amplification products are added to the MLFA strip, they are driven forward by capillary action, and magnetic signals and color appear at the control line (C line) or both the control and test lines (T line). The results can be read visually, or they can be detected automatically with a magnetic signal reader. iScience 2018 7, 170-179DOI: (10.1016/j.isci.2018.09.005) Copyright © 2018 The Author(s) Terms and Conditions

Figure 2 Effect of NaOH in the Direct PCR-MLFA System (A) Image of blood cells treated with physiological saline or NaOH. (B) Transmission electron microscopy images of GMNPs-anti-digoxin antibodies treated with ddH2O or direct PCR product. (C) Images and average size (diameter) of GMNPs-anti-digoxin antibodies under different treatment conditions. iScience 2018 7, 170-179DOI: (10.1016/j.isci.2018.09.005) Copyright © 2018 The Author(s) Terms and Conditions

Figure 3 Standard Curve for the Direct PCR-MLFA Detection Standard curve for the detection of the average magnetic signal at the T line relative to the respective WBC count in whole blood (A) or the oral epithelial cell density in (B) saliva, and (C) buccal swab samples. iScience 2018 7, 170-179DOI: (10.1016/j.isci.2018.09.005) Copyright © 2018 The Author(s) Terms and Conditions

Figure 4 Genotyping Results for Multiple Sample Types (the Magnetic Signal Value of the Wild-Type Was Defined as a Negative Value) (A) Genotyping results of 200 whole blood samples. (B) Genotyping results of 200 dried blood spot samples. (C) Genotyping results of 50 saliva samples. (D) Genotyping results of 50 buccal swab samples. iScience 2018 7, 170-179DOI: (10.1016/j.isci.2018.09.005) Copyright © 2018 The Author(s) Terms and Conditions