Volume 12, Issue 1, Pages 1-6 (July 2015)

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Presentation transcript:

Volume 12, Issue 1, Pages 1-6 (July 2015) Identification of Hemagglutinin Residues Responsible for H3N2 Antigenic Drift during the 2014–2015 Influenza Season Benjamin S. Chambers, Kaela Parkhouse, Ted M. Ross, Kevin Alby, Scott E. Hensley Cell Reports Volume 12, Issue 1, Pages 1-6 (July 2015) DOI: 10.1016/j.celrep.2015.06.005 Copyright © 2015 The Authors Terms and Conditions

Cell Reports 2015 12, 1-6DOI: (10.1016/j.celrep.2015.06.005) Copyright © 2015 The Authors Terms and Conditions

Figure 1 Genetically Distinct H3N2 Viruses Circulated during 2014–2015 Influenza Season in the United States (A) HA sequences deposited on the GISAID database were analyzed. All sequences deposited by United States laboratories were included in the analysis. Shown are the percentage of viruses that belong to each HA clade (as defined in the text). (B) The H3 structure (Protein Data Bank: 1HGG) is shown with residues from Table 1 shown in yellow. (C and D) ELISAs were completed with plates coated with VLPs containing A/Texas/50/2012-WT HA (A/TX/12-WT), A/Texas/50/2012-F159S HA (A/TX/12-F159S), or A/Port Chalmers/1/1973 HA (A/PC/73). ELISAs were completed with A/Texas/50/2012 ferret anti-sera (C) or the F49 mAb (D) that binds to a conserved region of the HA stalk. Shown are mean and SEM of triplicate samples. Data are representative of two independent experiments. Cell Reports 2015 12, 1-6DOI: (10.1016/j.celrep.2015.06.005) Copyright © 2015 The Authors Terms and Conditions