Endemic meningococcal disease in Cerdanyola, Spain, 1987–93: molecular epidemiology of the isolates of Neisseria meningitidis M. Esther Verdú, Pere Coll,

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Endemic meningococcal disease in Cerdanyola, Spain, 1987–93: molecular epidemiology of the isolates of Neisseria meningitidis M. Esther Verdú, Pere Coll, Dionisia Fontanals, Francesca March, Immaculada Pons, Isabel Sanfeliu, Guillem Prats Clinical Microbiology and Infection Volume 2, Issue 3, Pages 168-178 (December 1996) DOI: 10.1016/S1198-743X(14)65139-9 Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 Genetic relationships of electrophoretic types (ETs) of N. meningitidis strains. The dendrogram was generated by the average-linkage method of clustering from matrix of coefficients of pairwise genetic distance (based on seven enzyme loci) for 13 ETs. Strain number, antigenic phenotypes (APs), pulse types (PTs) and electrophoretic types (ETs) are indicated. Clinical Microbiology and Infection 1996 2, 168-178DOI: (10.1016/S1198-743X(14)65139-9) Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 2 PFGE separation of N. meningitidis genomic DNA. DNAs cleaved with the endonuclease NheI were separated into 16 different pulse types (PTs) by PFGE. (A) Lane 1, strain 912465 (PT A1); lane 2, strain 889638 (PT E); lane 3, strain 891529 (PT A4); lane 4, strain 9208301 (PT F); lane 5, strain 9327251 (PT A8); lane 6, strain 913128 (PT A7); lane 7, strain 9120240 (PT C); lane 8, strain 878736 (PT I1); lane 9, strain 913369 (PT I2); lane 10, strain 9315144 (PT II). (B) Lane 1, strain 904775 (PT A5); lane 2, strain 911643 (PT A2); lane 3, strain 871760 (PT A3); lane 4, strain 903943 (PT A6); lane 5, strain 9016914 (PT B). Lanes M, standards (lambda concatemer). Sizes (kb) of standard DNA molecules are indicated. The strain 9304432 (PT D) is not shown. Clinical Microbiology and Infection 1996 2, 168-178DOI: (10.1016/S1198-743X(14)65139-9) Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 3 Dendrogram showing similarities between the NheI pulse types of the 30 N. meningitidis strains on the basis of the results of PFGE analysis. The dendrogram was generated by the UPGMA method of clustering from matrix of Dice similarity coefficients. The similarity among the patterns is indicated below the dendrogram as a percentage. Strain number, antigenic phenotypes (APs), pulse types (PTs) and electrophoretic types (ETs) are also indicated. Clinical Microbiology and Infection 1996 2, 168-178DOI: (10.1016/S1198-743X(14)65139-9) Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 4 Similarity matrix of the closely or possibly related pulse types (PTs) of serogroup B isolates from Cerdanyola during 1987–93. The value of the Dice similarity coefficient (DSC) between 63% and 95% is depicted by three different tones in the matrix. Clinical Microbiology and Infection 1996 2, 168-178DOI: (10.1016/S1198-743X(14)65139-9) Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions