Increased expression of Interleukin-13 but not Interleukin-4 in cystic fibrosis patients Hans-Peter Hauber, Djalal Gholami, Gerhard Koppermann, Hans-Eberhard.

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Increased expression of Interleukin-13 but not Interleukin-4 in cystic fibrosis patients Hans-Peter Hauber, Djalal Gholami, Gerhard Koppermann, Hans-Eberhard Heuer, Andreas Meyer, Almuth Pforte Journal of Cystic Fibrosis Volume 2, Issue 4, Pages 189-194 (December 2003) DOI: 10.1016/S1569-1993(03)00091-2

Fig. 1 RT-PCR of IL-13 mRNA expression in BAL cells and PBM of patients and controls. Lanes 1–8: β-actin in BAL cells (1,2) and PBM (3,4) of two patients and in BAL cells (5,6) and PBM (7,8) of two control persons as internal standard. Lanes 9–16: IL-13 is expressed in BAL cells (9,10) and PBM (11,12) of the patients but only in low amounts in BAL cells (13,14) and PBM (15,16) of the control persons. Lenght of IL-13 transcript was 275 bp as expected. M: molecular weight marker. Journal of Cystic Fibrosis 2003 2, 189-194DOI: (10.1016/S1569-1993(03)00091-2)

Fig. 2 RT-PCR of IL-4 mRNA expression in BAL cells and PBM of patients and control persons. Lanes 1–8: β-actin in BAL cells (1,2) and PBM (3,4) of two patients and in BAL cells (5,6) and PBM (7,8) of two control persons as internal standard. Lanes 9–16: IL-4 is expressed in PBM of the patients (11,12) and the control persons (15,16) but not in BAL cells of the patients (9,10) or the control persons (13,14). Lenght of IL-4 transcript was 399 bp as expected. M: molecular weight marker. Journal of Cystic Fibrosis 2003 2, 189-194DOI: (10.1016/S1569-1993(03)00091-2)

Fig. 3 TNFα protein concentrations in PBM cell culture supernatants. Columns indicate mean values after 18 h of incubation (20.69 pg/ml, control) with rhIL-13 (16.25 pg/ml) and antiIL-13 (43.69 pg/ml) and after stimulation using LPS (760.82 pg/ml, control) with rhIL-13 (259.07 pg/ml) and antiIL-13 (818.15 pg/ml). *: P<0.05 vs. control. Journal of Cystic Fibrosis 2003 2, 189-194DOI: (10.1016/S1569-1993(03)00091-2)