Evaluation of assay performance with positive and negative binary reference sets Evaluation of assay performance with positive and negative binary reference.

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Evaluation of assay performance with positive and negative binary reference sets Evaluation of assay performance with positive and negative binary reference sets A, BReproducibility of LuTHy PPI mapping experiments with positive and negative reference sets. The scatter plots show the mean cBRET (A) and cLuC (B) ratios from two independent experiments (Exp 1 and Exp 2), and the calculated two‐tailed Pearson correlation coefficient is indicated; ***P < 0.001.CReceiver operating characteristic (ROC) analysis of cBRET (AUC = 0.80 ± 0.04) and cLuC (AUC = 0.72 ± 0.04) data determines the thresholds to define true positive binary interactions.DPerformance of BRET and LuC assays in benchmarking studies with hPRS and hRRS. Philipp Trepte et al. Mol Syst Biol 2018;14:e8071 © as stated in the article, figure or figure legend