PGC‐1α‐independent regulation of the slow‐twitch muscle fiber program by miR‐499 PGC‐1α‐independent regulation of the slow‐twitch muscle fiber program.

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PGC‐1α‐independent regulation of the slow‐twitch muscle fiber program by miR‐499 PGC‐1α‐independent regulation of the slow‐twitch muscle fiber program by miR‐499 Representative Western blot analysis of PGC‐1α protein expression in the gastrocnemius muscle from the indicated genotypes (n = 4 mice per group).Morphology of dissected gastrocnemius/soleus (asterisk) and tibialis anterior (TA) muscle.Expression of genes encoding slow‐twitch/fast‐twitch troponin and Sox6 (RT‐qPCR) in soleus (Sol), gastrocnemius (GC), and white vastus (WV) muscle from the indicated genotypes. For Sol, NTG, n = 10; 499Tg, n = 9; PGC‐1α mKO, n = 8; 499Tg/PGC‐1α mKO, n = 10. For GC and WV, n = 5 mice per group. *P < 0.05 (versus NTG), ‡P < 0.05 (versus 499Tg).Data information: Values represent the mean (± SEM) and are shown as arbitrary units (AU) normalized (= 1.0) to the value of the NTG control. P‐value was determined using one‐way ANOVA coupled to a Fisher's least‐significant difference (LSD) post hoc test.Source data are available online for this figure. Jing Liu et al. EMBO Mol Med. 2016;8:1212-1228 © as stated in the article, figure or figure legend