Volume 61, Issue 1, Pages (January 2002)

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Volume 61, Issue 1, Pages 141-147 (January 2002) Patterns of nephrin and a new proteinuria-associated protein expression in human renal diseases  Shi-Xuan Wang, Maria P. Rastaldi, Anu Pätäri, Heikki Ahola, Eija Heikkilä, Harry Holthöfer, M.D.  Kidney International  Volume 61, Issue 1, Pages 141-147 (January 2002) DOI: 10.1046/j.1523-1755.2002.00114.x Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 1 Immunoperoxidase staining of normal (a), membranous (b), membranoproliferative (c) and IgA nephropathy (d) with antinephrin antibodies. Distinct down-regulation of reactivity can be seen in all samples of diseased kidney. Kidney International 2002 61, 141-147DOI: (10.1046/j.1523-1755.2002.00114.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 2 Immunoperoxidase (a–g) and immunofluorescence (h) staining of different diagnostic kidney biopsies with monoclonal antibody (mAb)18C7. (a) Normal human kidney; (b) IgA glomerulonephritis; (c) membranous glomerulonephropathy; (d) focal segmental glomerulosclerosis; (e) cryoglobulinemic glomerulonephritis; (f) membranoproliferative glomerulonephritis; (g) systemic lupus erythematosus; (h) CNF (original magnification, ×200). Kidney International 2002 61, 141-147DOI: (10.1046/j.1523-1755.2002.00114.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 3 Western blotting of normal human kidney glomerular lysate by mAb 18C7. The clear band is seen at around 240 kD, while on the control lane using irrelevant mouse IgG instead of 18C7 no clear band is found. Molecular mass markers are shown on the right. Kidney International 2002 61, 141-147DOI: (10.1046/j.1523-1755.2002.00114.x) Copyright © 2002 International Society of Nephrology Terms and Conditions