Dopa oxidase – the perfect enzyme? Paul Beaumont / Kate Andrews Kath Crawford / Lorraine Bruce

What characteristics do you look for in an enzyme practical?

Robust Cheap Versatile Reliable ‘Living thing-based’ Easy to assay Quick Amenable to kinetic studies Open to investigations e.g. temperature, pH

Tyrosinase / dopa oxidase / catechol oxidase Adapted from http://mystrica.com/

Reaction

Materials As per instruction sheet.

The Assay Banana ~ 2.5 g + 5.0 mL cold H2O Centrifuge 2 min – enzyme extract Zero colorimeter at 470 nm In cuvette: 200 mL extract + 800 mL H2O Place in colorimeter Add 2.0 mL L-dopa solution and start stop-clock. Measure colour every 20 s (or so...)