Â. C. Volpini, M. J. Marques, S. Lopes dos Santos, G. L

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Leishmania identification by PCR of Giemsa-stained lesion imprint slides stored for up to 36 years Â.C. Volpini, M.J. Marques, S. Lopes dos Santos, G.L. Machado-Coelho, W. Mayrink, A.J. Romanha Clinical Microbiology and Infection Volume 12, Issue 8, Pages 815-818 (August 2006) DOI: 10.1111/j.1469-0691.2006.01422.x Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Representative polyacrylamide 10% w/v gel of PCR products obtained with primers specific for the conserved region of Leishmania mkDNA. Lanes: 1, reference strain of Leishmania (Leishmania) amazonensis IFLA/BR/1967/PH8; 2, reference strain of Leishmania (Viannia) braziliensis MHOM/BR/1975/M2903; 3 and 4, DNA from lesions of two patients with American cutaneous leishmaniasis; 5–12, restriction fragment length polymorphism fragments obtained after endonuclease digestion of the amplicons from lanes 1–4 with HaeIII (lanes 5–8) or ApaLI (lanes 9–12). MM, 25-bp molecular size ladder; NC, DNA-negative control. Clinical Microbiology and Infection 2006 12, 815-818DOI: (10.1111/j.1469-0691.2006.01422.x) Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions