Volume 15, Issue 5, Pages (May 2008)

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Volume 15, Issue 5, Pages 476-484 (May 2008) The Role of Cep15 in the Biosynthesis of Chloroeremomycin: Reactivation of an Ancestral Catalytic Function Andrew W. Truman, Qingzhi Fan, Marlene Röttgen, Evi Stegmann, Peter F. Leadlay, Jonathan B. Spencer Chemistry & Biology Volume 15, Issue 5, Pages 476-484 (May 2008) DOI: 10.1016/j.chembiol.2008.03.019 Copyright © 2008 Elsevier Ltd Terms and Conditions

Figure 1 Glycopeptides Whose Gene Clusters Contain a Gene Encoding a COG2120 Domain Protein (A) Structures of glycopeptides. Amino acids on chloroeremomycin are numbered aa 1 to aa 7. (B) Comparison of COG2120 genes (shaded dark gray) within glycopeptide gene clusters. Chemistry & Biology 2008 15, 476-484DOI: (10.1016/j.chembiol.2008.03.019) Copyright © 2008 Elsevier Ltd Terms and Conditions

Figure 2 N-Acetylglucosaminyl Pseudoaglycones Referred to in This Study Chemistry & Biology 2008 15, 476-484DOI: (10.1016/j.chembiol.2008.03.019) Copyright © 2008 Elsevier Ltd Terms and Conditions

Figure 3 Inactivation of orf2∗ in the Teicoplanin Gene Cluster (A) Removal of a 285 bp region from orf2∗. (B) Verification of deletion by PCR of the orf2∗ region: Left to right = Marker (Hyperladder I, Bioline); negative plasmid control (pQZ10); positive plasmid control (pQZ12); wild-type genomic DNA; Δorf2∗ genomic DNA. (C) Metabolite profile of Δorf2∗ mutant strain (selective ion monitoring for teicoplanin derivatives). Chemistry & Biology 2008 15, 476-484DOI: (10.1016/j.chembiol.2008.03.019) Copyright © 2008 Elsevier Ltd Terms and Conditions

Figure 4 Comparative Sequence Alignment of the Putatively Functionless Cep15 and Bal2 with Characterized Deacetylases Orf2∗ and Dbv21 Fully conserved residues have white text on a red background, and similar amino acids are framed in blue. Similar residues within a group are colored red and residues that significantly differ from one group to another have an orange background. Metal binding residues are denoted by triangles, the active site base by a star and substrate binding residues by circles. Chemistry & Biology 2008 15, 476-484DOI: (10.1016/j.chembiol.2008.03.019) Copyright © 2008 Elsevier Ltd Terms and Conditions

Figure 5 The Activity of the Reactivated Deacetylase Cep15 N164H Activity of Cep15 N164H with chloroeremomycin pseudoaglycone 3 monitored by LC/ESI-MS. Chemistry & Biology 2008 15, 476-484DOI: (10.1016/j.chembiol.2008.03.019) Copyright © 2008 Elsevier Ltd Terms and Conditions

Figure 6 Competitive Deacetylation of Pseudoaglycones 1 and 3 with Cep15 N164H As a result of overlapping peaks in the HPLC spectrum, the relative rates of deacetylation are observed using selective ion monitoring for the appropriate substrates and products. Chemistry & Biology 2008 15, 476-484DOI: (10.1016/j.chembiol.2008.03.019) Copyright © 2008 Elsevier Ltd Terms and Conditions