Rapid cellular luminal coverage of Dacron inferior vena cava prostheses in dogs by immediate seeding of autogenous endothelial cells derived from omental.

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Rapid cellular luminal coverage of Dacron inferior vena cava prostheses in dogs by immediate seeding of autogenous endothelial cells derived from omental tissue: Results of a preliminary trial Zhonggao Wang, MD *, Wei Du, MD *, Guang-di Li, MD *, Li-qun Pu, MD *, John B. Sharefkin, MD ** Journal of Vascular Surgery Volume 12, Issue 2, Pages 168-179 (August 1990) DOI: 10.1016/0741-5214(90)90105-J Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 Scanning electron micrograph of the luminal surface of a seeded graft after seeding but before implantation. Large numbers of seeded cells with poorly defined morphologic features appear to be clustered on the luminal surface and intermixed with a dense layer of fibrin coagulum. (Original magnification, × 1000.) Journal of Vascular Surgery 1990 12, 168-179DOI: (10.1016/0741-5214(90)90105-J) Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 A, Gross photograph of seeded Dacron inferior vena cava prosthesis removed 10 days after implantation. A smooth and comparatively thin pseudointima is present without evidence of gross thrombus. Cephalad end of prosthesis is at right of this photograph and at right of B.B, Gross photograph of control (unseeded) Dacron inferior vena cava prosthesis removed 10 days after implantation. A red thrombus with a grossly irregular surface covers most of the luminal surface of the prosthesis. Journal of Vascular Surgery 1990 12, 168-179DOI: (10.1016/0741-5214(90)90105-J) Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Scanning electron micrograph of a seeded graft removed for study 24 hours after implantation. Much of the surface is still covered by a mixed thrombus of fibrin strands and blood platelets with occasional red blood cells and white cells present. In addition, some partially flattened cells with endothelial-like morphologic characteristics, such as the cell labeled E in the figure, appear to be emerging on the surface and spreading. (Original magnification, × 1000.) Journal of Vascular Surgery 1990 12, 168-179DOI: (10.1016/0741-5214(90)90105-J) Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 A, and B, Scanning electron micrographs taken of the surface of seeded grafts removed for study at 5 days (A) and at 10 days after implantation (B). The intima appears to have acquired a completely confluent covering of flattened endothelial-like cells aligned parallel to the axis of blood flow with closely apposed borders and shingle-like overlaps of upstream cells over adjacent downstream ones typical of endothelial monolayers. In Fig. B the flow direction is from right to left; the band of more spindle-shaped cells crossing the upper half of the figure may represent endothelial cell alignment in response to shear at a boundary between zones of different flow direction.39,40 (Original magnifications, (A) × 1000, (B) × 200.) Journal of Vascular Surgery 1990 12, 168-179DOI: (10.1016/0741-5214(90)90105-J) Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 5 Scanning electron micrograph of control unseeded graft removed 10 days after implantation. In contrast to the appearance of seeded grafts in Fig. 4 A and B, the surface remains covered by a mixed thrombus with platelets, some red cells, and fibrin strands still present. (Original magnification, × 1000.) Journal of Vascular Surgery 1990 12, 168-179DOI: (10.1016/0741-5214(90)90105-J) Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 6 Transmission electron micrograph of the cells lining the luminal surface of a seeded graft removed 10 days after implantation. The luminal lining cells facing the lumen at upper right show endothelial cell-like ultrastructural features, which include microvilli, attenuated peripheral cytoplasm, and pinocytotic vesicles. The cell type beneath the luminal lining cells is not identified but has a segmented irregular nuclear shape and is surrounded by amorphous matrix-like material containing collagen fibers. (Original magnification, × 4000.) Journal of Vascular Surgery 1990 12, 168-179DOI: (10.1016/0741-5214(90)90105-J) Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions