Volume 12, Issue 11, Pages (November 2005)

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Volume 12, Issue 11, Pages 1163-1168 (November 2005) Investigating β-Hydroxyenduracididine Formation in the Biosynthesis of the Mannopeptimycins Brad Haltli, Ying Tan, Nathan A. Magarvey, Melissa Wagenaar, Xihou Yin, Michael Greenstein, John A. Hucul, T. Mark Zabriskie Chemistry & Biology Volume 12, Issue 11, Pages 1163-1168 (November 2005) DOI: 10.1016/j.chembiol.2005.09.013 Copyright © 2005 Elsevier Ltd Terms and Conditions

Figure 1 Structure of Natural Mannopeptimycins Produced by S. hygroscopicus NRRL 30439 The L-βhEnd residue is blue, and the D-βhEnd residue is red. Chemistry & Biology 2005 12, 1163-1168DOI: (10.1016/j.chembiol.2005.09.013) Copyright © 2005 Elsevier Ltd Terms and Conditions

Figure 2 Sequence Alignment of Key Motifs Found MppO and Related Proteins ClustalW alignment of (A) His-1, (B) His-3, and (C) His-2 motifs of MppO and its homologs. Results are shown for AsnO from Streptomyces coelicolor [17], SttL from Streptomyces rochei F20 (direct deposit to the data bank by F. Malpartida, accession number CAB67713), VioC from Streptomyces vinaceus [7, 8], MppO from Streptomyces hygroscopicus NRRL 30439 [10], and CS1 and CS2 from Streptomyces clavuligerus [9]. Invariant residues are indicated with an asterisk. Positions with conservative substitutions are indicated with a colon. Positions with semiconservative substitutions are indicated with a period. Residues proven to be essential for CS2 activity are shaded. The residue proposed by this study to affect activity of MppO is in bold type. Chemistry & Biology 2005 12, 1163-1168DOI: (10.1016/j.chembiol.2005.09.013) Copyright © 2005 Elsevier Ltd Terms and Conditions

Figure 3 LC/MS Comparison of Putative Dideoxy-MPP-γ Isolated from Mutant WPN105 to Authentic Dideoxy-MPP-γ and -δ Standards (A) Dideoxy-MPP-γ and -δ standards. (B) Putative dideoxy-MPP-γ isolated from mutant WPN105. (C) Coinjection of (A) and (B). Chemistry & Biology 2005 12, 1163-1168DOI: (10.1016/j.chembiol.2005.09.013) Copyright © 2005 Elsevier Ltd Terms and Conditions

Figure 4 Heterologous Expression and Characterization of His6-MppO (A) SDS-PAGE analysis of the expression in E. coli and purification of His6-MppO. Lane 1, soluble protein; lane 2, protein eluted from Co2+ affinity column in 150 mM buffered imidazole; lanes 3 and 4, protein eluted from Co2+ affinity column in 250 mM buffered imidazole. The calculated MW of His6-MppO is 38.6 kDa. (B) RP-HPLC analysis of the MppO assay: (i) complete MppO assay; (ii) assay with boiled MppO. (C) RP-HPLC analysis of the substrate stereospecificity of MppO: (i) control MppO assay with untreated D,L-End; (ii) MppO assay in which the D,L-End was preincubated with D-amino acid oxidase for 1 hr; (iii) MppO assay in which the D,L-End was preincubated with L-amino acid oxidase for 1 hr. (D) Proposed reaction catalyzed by MppO. Chemistry & Biology 2005 12, 1163-1168DOI: (10.1016/j.chembiol.2005.09.013) Copyright © 2005 Elsevier Ltd Terms and Conditions