A novel role for the LisRK two-component regulatory system in listerial osmotolerance  R.D. Sleator, C. Hill  Clinical Microbiology and Infection  Volume 11, Issue 8, Pages 599-601 (August 2005) DOI: 10.1111/j.1469-0691.2005.01176.x Copyright © 2005 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Growth in brain–heart infusion (BHI) broth supplemented with NaCl 8% w/v. LO28 wild-type (solid circles), LO28ΔlisK (open circles) and LO28ΔlisK::P44htrA (LO28ΔlisK harbouring pNZ44htrA, which expresses a promoterless copy of htrA under the transcriptional control of the constitutive Lactococcus lactis promoter P44) (solid triangles). Following inoculation (2% v/v) with an overnight culture, OD595 readings were taken every hour for 40 h using a microplate reader. Each point represents the mean of three independent experiments. Results were verified by plate counts. Clinical Microbiology and Infection 2005 11, 599-601DOI: (10.1111/j.1469-0691.2005.01176.x) Copyright © 2005 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Transcriptional analysis of htrA using reversetranscriptase PCR. Total RNA was extracted from stationary-phase cultures of LO28 wild-type, LO28ΔlisK and LO28Δlisk::P44htrA. RNA was converted to cDNA and PCR was performed using the htrA-specific primers HtrAR and HtrA-out [4]. Products generated after 15 PCR cycles are shown. In all cases, control PCRs using the rrn primers U141 and L142 [10] were used to confirm that cDNA levels of samples to be compared were equal. Clinical Microbiology and Infection 2005 11, 599-601DOI: (10.1111/j.1469-0691.2005.01176.x) Copyright © 2005 European Society of Clinical Infectious Diseases Terms and Conditions