Fertility and Sterility

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Fertility and Sterility An alternative method of deriving embryonic stem cell-like clones by aggregation of diploid cells with tetraploid embryos Meeyoung Cho, B.Sc., Mi Jang, B.Sc., Eun Ju Lee, Ph.D., Jae Yong Han, Ph.D., Jeong Mook Lim, Ph.D., D.V.M. Fertility and Sterility Volume 85, Pages 1103-1110 (April 2006) DOI: 10.1016/j.fertnstert.2005.10.024 Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 Characterization of embryonic stem (ES)-like cells derived from the inner cell mass of tetraploid blastocysts that were aggregated with E14 diploid cells. Alkaline phosphatase (AP; A–C), anti-SSEA-1 (D–F), anti-SSEA-3 (G–I), anti-SSEA-4 (J–L), and Oct-4 (M–O) were used for the characterization. Two batches of ES-like cells (SNU-Tet1 and SNU-Tet3) were characterized, and as the positive control, a commercial ES cell line was employed. The results showed that both tetraploid-derived ES-like cells were positive for anti-SSEA-1 antibody, AP, and Oct-4, while negative for anti-SSEA-3 and anti-SSEA-4 antibodies, which was identical to the reactivity of the commercial ES cell line (scale bar, 150 μm). Cho. Alternative establishment of stem cell. Fertil Steril 2006. Fertility and Sterility 2006 85, 1103-1110DOI: (10.1016/j.fertnstert.2005.10.024) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Microsatellite analysis of live births derived from the transfer of tetraploid blastocysts aggregated with E14 diploid cells. A total of six markers (D10Mit035, D18Mit060, D14Mite082, D14Mite096, D09Mit206, and D11Mite242) were provided, and a comparison was made among the E14 cells, surrogate mothers, live births (tail cells), SNU-tet1, and SNU-tet3. The results showed that the DNA spectra of the live births, SNU-tet1, and SNU-tet3 were identical with those of the E14 diploid cells, which differed from those of the surrogate mothers. Cho. Alternative establishment of stem cell. Fertil Steril 2006. Fertility and Sterility 2006 85, 1103-1110DOI: (10.1016/j.fertnstert.2005.10.024) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions