Identification of IgE- and IgG-binding epitopes on αs1-casein: Differences in patients with persistent and transient cow's milk allergy Pantipa Chatchatee,

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Presentation transcript:

Identification of IgE- and IgG-binding epitopes on αs1-casein: Differences in patients with persistent and transient cow's milk allergy Pantipa Chatchatee, MD, Kirsi-Marjut Järvinen, MD, Ludmilla Bardina, MS, Kirsten Beyer, MD, Hugh A. Sampson, MD Journal of Allergy and Clinical Immunology Volume 107, Issue 2, Pages 379-383 (February 2001) DOI: 10.1067/mai.2001.112372 Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 1 Cumulative OD scores for IgE for each of the synthetic decapeptides of αs1-casein. According to the known AA sequence, 96 overlapping decapeptides were synthesized on a cellulose-derivatized membrane. Sera from 24 children with milk allergy were used to identify IgE-binding epitopes. Numbers on top of bars represent the actual AA. Journal of Allergy and Clinical Immunology 2001 107, 379-383DOI: (10.1067/mai.2001.112372) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 2 AA sequence of αs1-casein. IgE-binding epitopes are underlined; thick lines represent major epitopes, and broken lines represent the minor ones. IgG-binding epitopes are shown in italics, and the major epitopes are shown in bold. Asterisks depict the location of the 2 epitopes with the following sequence homology: X1EIVPNSX2EX3. Journal of Allergy and Clinical Immunology 2001 107, 379-383DOI: (10.1067/mai.2001.112372) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 3 Selected part of the Spot membrane showing Spot number 35, corresponding to the third epitope (AA 69-78), and Spot number 89, corresponding to the decapeptide 177-168 in epitope 9 (AA 173-194). Shown is the IgE labeling with sera from 3 patients with persistent CMA at an early phase of the disease and several years later. The sera used were obtained at the following ages: patient 1 at 2 and 9 years, patient 2 at 3 and 14 years, and patient 3 at 1 and 10 years of age. Journal of Allergy and Clinical Immunology 2001 107, 379-383DOI: (10.1067/mai.2001.112372) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 4 Cumulative OD scores for IgG for each of the synthetic decapeptides of αs1-casein. According to the known AA sequence, 96 overlapping decapeptides were synthesized on a cellulose-derivatized membrane. Sera from 24 children with milk allergy were used to identify IgG-binding epitopes. Numbers on top of bars represent the actual AA. Journal of Allergy and Clinical Immunology 2001 107, 379-383DOI: (10.1067/mai.2001.112372) Copyright © 2001 Mosby, Inc. Terms and Conditions