Analysis of the Biliary Transcriptome in Experimental Biliary Atresia Elisa Carvalho, Cong Liu, Pranavkumar Shivakumar, Gregg Sabla, Bruce Aronow, Jorge A. Bezerra  Gastroenterology  Volume 129, Issue 2, Pages 713-717 (August 2005) DOI: 10.1053/j.gastro.2005.05.052 Copyright © 2005 American Gastroenterological Association Terms and Conditions

Figure 1 (A) Data filtering and 2-way hierarchic cluster analysis of the biliary transcriptome identify 359 genes that differ at least 2-fold in rotavirus (RV)-challenged mice when compared with normal saline (NS) controls. The levels of gene expression are depicted as color variation from red (high expression) to green (low expression); each column represents the data from a microarray hybridized with a complementary RNA pool from 2–3 mice. (B) Number of genes from A that are up-regulated in RV mice above NS controls, and grouped into specific biological functions at 3, 7, and 14 days after RV or NS injection. Gastroenterology 2005 129, 713-717DOI: (10.1053/j.gastro.2005.05.052) Copyright © 2005 American Gastroenterological Association Terms and Conditions

Figure 2 Histologic cross-sections of the extrahepatic bile duct after rotavirus (RV) challenge showing mild inflammatory cell infiltration at 3 days, and pronounced cholangitis and injury to duct epithelium (arrows) at 7 days (also see Supplementary Figure 1). The histologic abnormalities are associated with the temporal expression of a hierarchic network of IFN responsive factors (Irf7 and Irf9), followed by IFN-γ and IFN-γ–regulated genes. The increased levels of expression for these genes were validated by real-time polymerase chain reaction (Supplementary Table 4). Black bar, 100 μm. Gastroenterology 2005 129, 713-717DOI: (10.1053/j.gastro.2005.05.052) Copyright © 2005 American Gastroenterological Association Terms and Conditions