Reference gene study for forensic body fluid identification

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Presentation transcript:

Reference gene study for forensic body fluid identification O.A. Afolabi, A.D. Roeder, A. Iyengar, S. Hadi Forensic Science International: Genetics Supplement Series Volume 5, Pages e167-e169 (December 2015) DOI: 10.1016/j.fsigss.2015.09.067 Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Amplification plots of ACTB (blue), RPS 29 (orange), TEF (red), B2M (green) and UCE (pink) on five body fluid samples; A: blood, B: vaginal secretion, C: menstrual blood, D: saliva, E: semen at 25pg, 50pg, 100pg, 500pg, 1ng and 2ng input RNA concentrations. Each reaction comprises donors’ samples (in duplicate) at different input RNA concentrations. Forensic Science International: Genetics Supplement Series 2015 5, e167-e169DOI: (10.1016/j.fsigss.2015.09.067) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 Histogram plot. Expression stability of RNA stains in over 6 month old samples (a) blood, (b) semen, (c) saliva, (d) menstrual blood, (e) vaginal secretion, with no modification made to the storage conditions to control RNA degradation. All markers except TEF were expressed in at least 80% of the analysed samples for each body fluid. Forensic Science International: Genetics Supplement Series 2015 5, e167-e169DOI: (10.1016/j.fsigss.2015.09.067) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions