BDA20, a major bovine dander allergen characterized at the sequence level, is Bos d 2 Jaakko Rautianinen, MSc, Marho Rytkönen, MSc, Tuomas Virtanen, MD,

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Presentation transcript:

BDA20, a major bovine dander allergen characterized at the sequence level, is Bos d 2 Jaakko Rautianinen, MSc, Marho Rytkönen, MSc, Tuomas Virtanen, MD, Jaana Pentikäinen, PhD, Thomas Zeiler, MD, Rauno Mäntyjärvi, MD Journal of Allergy and Clinical Immunology Volume 100, Issue 2, Pages 251-252 (August 1997) DOI: 10.1016/S0091-6749(97)70232-X Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 1 Reactivity of antibodies in immunoblotting against bovine dander extract (lanes 1 to 3), nBDA20 purified from dander extract (lanes 4 and 5), and rBDA20 (lanes 6 to 10). Immunoblotting with serum IgE of patient with cow dander–induced asthma (diluted 1:20) (lane 1), with anti-BDA20 monoclonal antibody (culture supernatant dilution 1:500) (lanes 2 and 4), and with rabbit anti-Bos d 2 serum (diluted 1:25,000) (lanes 3 and 5). In the inhibition test, anti-Bos d 2 serum (final dilution, 1:50,000) was allowed to react with nBDA20 (concentration, 100 μg/ml; lane 6), with rBDA20 (concentration, 150 μg/ml; lane 7), and with human serum albumin (concentration, 100 μg/ml; lane 8) before immunoblotting against rBDA20. Serum reactivity without inhibition (lane 9) and conjugate control (no primary antibody) (lane 10) are also shown. Journal of Allergy and Clinical Immunology 1997 100, 251-252DOI: (10.1016/S0091-6749(97)70232-X) Copyright © 1997 Mosby, Inc. Terms and Conditions