Volume 10, Issue 9, Pages (September 2002)

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Volume 10, Issue 9, Pages 1261-1272 (September 2002) Gene Sequence and the 1.8 Å Crystal Structure of the Tungsten-Containing Formate Dehydrogenase from Desulfovibrio gigas  Hans Raaijmakers, Sofia Macieira, João M Dias, Susana Teixeira, Sergey Bursakov, Robert Huber, José J.G Moura, Isabel Moura, Maria J Romão  Structure  Volume 10, Issue 9, Pages 1261-1272 (September 2002) DOI: 10.1016/S0969-2126(02)00826-2

Figure 1 Amino Acid Sequence Alignment of the DgW-FDH Large Subunit with Its Structural Neighbors FDHA_DEGIG, DgW-FDH α subunit, SWALL accession number Q934F5; NAPA_DEDES, NAP, SWALL accession number P88186; FDHF_ECOLI, FDH-H, SWISS-PROT accession number P07658. Conserved residues, light blue; cysteines involved in iron-sulfur cluster binding, dark yellow; selenocysteine, magenta. The secondary structural elements, β strands (→), and α helices (■) are colored according to the domains to which they belong. Domain I, red; domain II, green; domain III, yellow; domain IV, blue. ▾, signal peptide cleavage site. The residue numbering refers to the FDHA_DEGIG sequence and starts at the first residue of the mature protein. This figure was prepared with the programs PILEUP, in the Wisconsin Package Version 10.0 (Genetics Computer Group [GCG], Madison, WI), and ALSCRIPT [39]. Structure 2002 10, 1261-1272DOI: (10.1016/S0969-2126(02)00826-2)

Figure 2 Superposition of the Large Subunit of DgW-FDH in Blue with the Structure of the Periplasmic Nitrate Reductase from D. desulfuricans ATCC 27774 Represented in Green The rmsd for the superposition of 636 amino acid residues is 2.0 Å. The structure of FDH-H from E. coli [13] (red) and the corresponding rmsd for 659 amino acids of DgW-FDH is 2.1 Å. Structure 2002 10, 1261-1272DOI: (10.1016/S0969-2126(02)00826-2)

Figure 3 Stereo Representation of the Overall Structure of DgW-FDH from D. gigas The small domain (214 amino acid residues) is represented in purple, and the large domain (977 amino acid residues) is colored after its domain classification. Domain I (red) corresponds to residues 9–68, 575–603, and 647–734; domain II (green) contains residues 69–157, 410–574, and 604–646; domain III (yellow) is defined by residues 157–409; domain IV (blue) is defined by residues 735–977. Domain IV can be divided into two subdomains, IVa (residues 735–795) and IVb (residues 796–977). The insertions (relative to the homologous NAP and FDH-H structures) present in several domains are shown in gray. (B) Representation of the four “exploded” domains of the large 977 aa subunit. (C) Comparison of the domain organization of DgW-FDH, D. desulfuricans NAP, and E. coli FDH-H. The insertions in DgW-FDH are depicted as dashed areas. (D) Stereo Cα trace numbered every 20 residues for domains II and III (top). (E) Stereo Cα trace numbered every 20 residues for domains I and IV and for the small subunit (below). Structure 2002 10, 1261-1272DOI: (10.1016/S0969-2126(02)00826-2)

Figure 4 Schematic Representation of the Two Pyranopterin Guanosine Dinucleotide (MGD) Cofactors and the Hydrogen Bonding Interaction with Surrounding Residues and Buried Water Molecules Hydrogen bonds were identified using CONTACT from the CCP4 package [36] and TURBO-FRODO [37]. Structure 2002 10, 1261-1272DOI: (10.1016/S0969-2126(02)00826-2)

Figure 5 Small Subunit of DgW-FDH (A) Overall structure of the small subunit, as cartoon and stereo Cα trace. Domain A (red), 1–72 and 137–165; domain B (green), 73–136 (71–72 and 137 can be assigned to both domains A and B); domain C (yellow), 166–214. (B) The ferredoxin-like domains A (red) and B (green) can be superimposed to each other with an rmsd of 1.0 Å for 31 Cα atoms plus 8 atoms from the [4Fe-4S] cluster or an rmsd of 1.1 Å for 132 main chain atoms. The domains are related by a proper rotation. (C) Ferredoxin (Protein Data Bank entry 1blu; blue) has been superimposed on domain A (red) with an rmsd of 0.99 Å for 35 Cα atoms plus 16 [4Fe-4S] atoms. Domain B, green. Structure 2002 10, 1261-1272DOI: (10.1016/S0969-2126(02)00826-2)

Figure 6 Representation of the Tungsten Catalytic Site of DgW-FDH, Superimposed with the Final 2Fo − Fc Electron Density Map Contoured at 1.0 σ Surrounding residues important for catalysis are also included: SeCys158 (bound to the W atom [black sphere]), His159, and Arg407. To the left is Lys56, which makes a hydrogen bond to the exocyclic NH2 of MGD 1002. Structure 2002 10, 1261-1272DOI: (10.1016/S0969-2126(02)00826-2)

Figure 7 How DgW-FDH Works (A) Diagram of DgW-FDH cofactors and GRASP [38] electrostatic-potential surface plot contoured for positive charges (blue) and negative charges (red). The top cleft is lined with basic amino acids and provides an excellent entry point for the formate. At the W/Se center, the formate is split into CO2, H+, and two electrons. To the left are the four [4Fe-4S] clusters. Counting at increasing distance from W: [4Fe-4S] 1 (large subunit) Cys17-xx-20-xxx-24-xn-54; [4Fe-4S] 2 (small subunit) Cys11-xx-Cys14-xx-Cys17-xn-156; [4Fe-4S] 3 Cys21-xn-Cys137-xx-Cys140-xn-152; [4Fe-4S] 4 Cys72-xx-Cys75-xxxx-Cys80-xn-Cys120. There would be space and the correct fold for a fifth [4Fe-4S] if the residues Ala84-xn-Leu110-xx-Tyr113-xx-Val116 were mutated to Cys. (B) A chain of water and protonable residues provides a channel from the reaction center to the surface of the FDH. The orientation of the picture is similar to that of Figure 7A, middle and left. (C) More or less perpendicular to the formate entry and the proton channel is channel lined with aromatic and hydrogen bond-donating residues. Putative CO2 binding sites are indicated with sticks between observed water molecules (red). The picture has been rotated ∼20° around the horizontal axis and ∼35° around the vertical axis relative to Figure 7B. Structure 2002 10, 1261-1272DOI: (10.1016/S0969-2126(02)00826-2)