Human isolates of Aeromonas possess Shiga toxin genes (stx1 and stx2) highly similar to the most virulent gene variants of Escherichia coli A. Alperi,

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Human isolates of Aeromonas possess Shiga toxin genes (stx1 and stx2) highly similar to the most virulent gene variants of Escherichia coli A. Alperi, M.J. Figueras Clinical Microbiology and Infection Volume 16, Issue 10, Pages 1563-1567 (October 2010) DOI: 10.1111/j.1469-0691.2010.03203.x Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Examples of PCR amplifications demonstrating the presence of stx genes in Aeromonas strains. 25 µL of PCR product electrophoresed in 1% agarose gel. (a) stx1 strong and faint bands. Lane 1, 875c; lane 2, 883c; lane 3, 885c; lane 4, 887c; lane 5, 194c; lane 6, 361c; lane 7, 191c; lane 8, 24c. (b) stx2 faint bands. Lane 1, 875c; lane 2, 819c; lane 3, 819c (duplicate PCR product). (c) stx1 amplified from plasmid DNA. Lane 1, 535c. Lane C+, positive control Escherichia coli O157:H (CECT 4076). Lane C-, negative control (Milli-Q water as template DNA; Millipore, Billerica, MA, USA). Lane M, Ladder 100 bp. Aeromonas caviae strains: 535c, 819c, 875c, 883c and 885c; Aeromonas hydrophila strains: 24c, 191c, 194c and 887c; Aeromonas salmonicida strain 361c. Clinical Microbiology and Infection 2010 16, 1563-1567DOI: (10.1111/j.1469-0691.2010.03203.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Phylogenetic analysis of nucleotide sequence fragments (350 bp) of stx1 genes (a) and (406-bp) of stx2 genes (b) of Aeromonas and other enterobacteria. Phylogenetic trees were constructed with the neighbour-joining method using Kimura's two-parameter model. Bootstrap values greater than 50 from 1000 replicate trees are reported at the nodes. The scale bar indicates evolutionary distance of 0.01 and 0.05 nucleotide substitutions per site in (a) and (b), respectively. All numbers and accession numbers are given as cited in the GenBank database. Clinical Microbiology and Infection 2010 16, 1563-1567DOI: (10.1111/j.1469-0691.2010.03203.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions