Genetic and pharmacologic inactivation of KSR1 radiosensitizes A431 cells in vitro to the lethal effect of ionizing radiation. Genetic and pharmacologic.

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Genetic and pharmacologic inactivation of KSR1 radiosensitizes A431 cells in vitro to the lethal effect of ionizing radiation. Genetic and pharmacologic inactivation of KSR1 radiosensitizes A431 cells in vitro to the lethal effect of ionizing radiation. A and B, clonogenic survival assays were conducted in A431-pTRE-KSR-Tet-Off cell lines (A) or in A431 cells that received KSR1 AS-ODN treatment (B). Radiation dose curves were constructed as in Materials and Methods. Dq, shoulder region; D0, the slope of the survival curve and a quantitative measure of intrinsic radiosensitivity; D0.1, radiation dose yields 1 log scale kill. C, radiation clonogenic response in A431-pTRE and A431-KSR-S cells treated with 50 nmol/L of AG1478. D, radiation clonogenic response in A549 NSCLC cells treated with 200 nmol/L of AG1478 (i) and determination of EGFR expression and IR-induced EGFR activation by ELISA (ii). Error bars, mean ± SE. Six to nine replicates were included for each experimental point to construct clonogenic survival curves. Hongyan Xiao et al. Mol Cancer Ther 2010;9:2724-2736 ©2010 by American Association for Cancer Research