Fig. 7 DMN-Tre labeling of Mycobacterium tuberculosis is inhibited by tuberculosis drug cocktail, unlike auramine staining. DMN-Tre labeling of Mycobacterium.

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Fig. 7 DMN-Tre labeling of Mycobacterium tuberculosis is inhibited by tuberculosis drug cocktail, unlike auramine staining. DMN-Tre labeling of Mycobacterium tuberculosis is inhibited by tuberculosis drug cocktail, unlike auramine staining. (A) Microscopy analysis of Mycobacterium tuberculosis (Mtb) cells incubated with 100 μM DMN-Tre for 2 hours. (B) Flow cytometry MFI analysis of Mtb cells incubated with DMN-Tre for the indicated times: 0.5, 1, 2, and 3 hours or overnight (~16 hours). Error bars denote three biological replicates. (C and D) Microscopy analysis of control and drug-treated Mtb cells labeled with 100 μM DMN-Tre overnight (C) or stained with the auramine-based Fluorescent Stain kit for mycobacteria (05151, Sigma-Aldrich) (D). Drug cocktail contents: ethambutol (1 μg/ml), rifampicin (0.2 μg/ml), SQ109 (10 μg/ml), and isoniazid (10 μg/ml) in 7H9 medium. Images were collected in the DIC or FITC/GFP (for DMN and Auramine fluorescence) channels of a Nikon A1R confocal microscope. Cells stained with auramine were given an orange pseudocolor. Scale bars, 5 μm. In (B), data are means ± SEM from at least two independent experiments. Data were analyzed by one-way ANOVA test (*P < 0.05, ***P < 0.001, and ****P < 0.0001). Mireille Kamariza et al., Sci Transl Med 2018;10:eaam6310 Published by AAAS