Phylogenetic tree of 38 Pseudomonas type strains, based on the V3-V5 region sequence of the 16S rRNA gene (V3 primer, positions 442 to 492; and V5 primer,

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Phylogenetic tree of 38 Pseudomonas type strains, based on the V3-V5 region sequence of the 16S rRNA gene (V3 primer, positions 442 to 492; and V5 primer, positions 822 to 879 [numbered according to the E. coli 16S rRNA gene map]). Phylogenetic tree of 38 Pseudomonas type strains, based on the V3-V5 region sequence of the 16S rRNA gene (V3 primer, positions 442 to 492; and V5 primer, positions 822 to 879 [numbered according to the E. coli 16S rRNA gene map]). The strains selected have full-genome sequences available through public databases. The V3-V5 sequence primers (212) were aligned to each genome by using DNAstar SeqBuilder software. A maximum likelihood tree was calculated in the online version of MAFFT (209, 210) and visualized with the software program Archaeopteryx (211). The confidence intervals after 1,000 bootstrap resamplings are indicated in red, and the branch distances are indicated in black. The bar indicates sequence divergence. Brittan S. Scales et al. Clin. Microbiol. Rev. 2014; doi:10.1128/CMR.00044-14