A novel forensic DNA profiling technique for protected species S. Ciavaglia, S. Donnellan, S. Tobe, J. Henry, A. Linacre  Forensic Science International: Genetics Supplement Series  Volume 5, Pages e258-e260 (December 2015) DOI: 10.1016/j.fsigss.2015.09.103 Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Amplification of MsF14 at varying efficacy: (a) full, (b) ∼0.63, (c) ∼0.35 and (d) weak amplification of 3.4 allele. Forensic Science International: Genetics Supplement Series 2015 5, e258-e260DOI: (10.1016/j.fsigss.2015.09.103) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 Heat map of amplification efficacy for each individual by geographic origin of sample. Red indicates minimal amplification and green indicates full amplification. In some cases MsF14 amplified more strongly than MsF31, indicated by blue. Circled individuals were fixed for the 3.4 allele, which amplified consistently weakly, between 0.01 and 0.1 of MsF31 alleles. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Forensic Science International: Genetics Supplement Series 2015 5, e258-e260DOI: (10.1016/j.fsigss.2015.09.103) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

Fig. 3 Sequencing of alleles shows underlying target motif AAGTT. Second repeat motif TGAACC can be seen in the reverse primer sequence and flanking region. Forensic Science International: Genetics Supplement Series 2015 5, e258-e260DOI: (10.1016/j.fsigss.2015.09.103) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions