Loss of Chk2 DNA damage signaling does not restore embryogenesis in vas mutant flies. Loss of Chk2 DNA damage signaling does not restore embryogenesis.

Slides:

Advertisements

Similar presentations

An analysis of Plk4 centriolar dynamics.

Advertisements

Phenotypes of GATA‐1.05/Y germline mutant embryos expressing wild‐type or mutant GATA‐1 transgenes. Phenotypes of GATA‐1.05/Y germline mutant embryos expressing.

Steroid Signaling Establishes a Female Metabolic State and Regulates SREBP to Control Oocyte Lipid Accumulation Matthew H. Sieber, Allan C. Spradling

Analysis of the deep-sequencing data.

PCS1 functions in gametogenesis and embryogenesis.

Phylogenetic Relationships and Expression Pattern of ZmbZIP22.

WT Segregant * Ds * * Figure S1. Fatty acid methyl esters of seed triacylglycerols for a wild-type segregant (WT Segregant) and transposon-tagged line.

(A) ZR75-1 cells were treated with 1 μM 5-aza-2′-deoxycytidine (5-Aza) for 4 d. (A) ZR75-1 cells were treated with 1 μM 5-aza-2′-deoxycytidine (5-Aza)

Volume 37, Issue 3, Pages (May 2016)

Volume 27, Issue 19, Pages e3 (October 2017)

Expression of GFP‐Aub transgenes in GSCs, and GSC loss phenotype in armi mutant Expression of GFP‐Aub transgenes in GSCs, and GSC loss phenotype in armi.

Disrupted dynamics of early stages of differentiation perturbs neural cell fate specification. Disrupted dynamics of early stages of differentiation perturbs.

Germline Stem Cell Heterogeneity Supports Homeostasis in Drosophila

Maternally transmitted transposon RNAs cause DNA double-strand breaks and nuclear damage in progeny embryos. Maternally transmitted transposon RNAs cause.

Loss of Grk2 impairs Hh signal transduction in zebrafish

Transposons invade maternal genome in vas, mnk double mutant flies.

Fig. 2 Fas controls IL-1RA–sEV secretion in murine MSCs.

Expression analysis of MC and JOINTLESS

MED12 association with ncRNA-a is disrupted by loss of CARM1 and TDRD3

Silencing of transposon RNAs during oogenesis is essential for embryonic development. Silencing of transposon RNAs during oogenesis is essential for embryonic.

BDNF expression in the cerebellum and brain stem region.

Cbl protein levels in ago3, twin, and armi mutant GSCs

Mutants for UVH6 and MED14 are impaired in heat stress–induced release of silencing. Mutants for UVH6 and MED14 are impaired in heat stress–induced release.

Maternally Inherited Stable Intronic Sequence RNA Triggers a Self-Reinforcing Feedback Loop during Development Mandy Li-Ian Tay, Jun Wei Pek Current.

Ismail Osman, Jun Wei Pek Stem Cell Reports

The CHCH domain is necessary for mitochondrial import of CHCHD10

Loss of FZR-1 results in elevated SAS-5 levels.

Identification of the uvh6-4 mutation.

GSC tumor phenotype in ago3 mutant germaria

Volume 44, Issue 4, Pages (November 2011)

ILK knockdown decreases mTOR signaling in PKD kidneys.

Nuclear DNA signal is altered in Chd5-deficient NSCs.

ACF1 loss perturbs gene expression in early embryos.

Dnmt2 mutants show delayed immune responses and Dnmt2–EGFP relocalizes and interacts with DCV RNA during infection. Dnmt2 mutants show delayed immune responses.

Silencing Ifngr1 in the CMT167 line confers decreased response to IFNγ in vitro and in vivo. Silencing Ifngr1 in the CMT167 line confers decreased response.

Notch1 loss alters the expression of the diffusible ligands Kitl and Tgfβ. Notch1 loss alters the expression of the diffusible ligands Kitl and Tgfβ. (A)

dany, tTAF and tMAC genes do not cross-regulate each other.

Fig. 4. Non-autonomous rescue of puc expression in DME cells

Fig. 6. Chk1-S173A reduces the viability of DNA polymerase epsilon mutant cells. Chk1-S173A reduces the viability of DNA polymerase epsilon mutant cells.

Synergistic activity of LMO-2, SCL and GATA-1 expression in whole embryos. Synergistic activity of LMO-2, SCL and GATA-1 expression in whole embryos. (Top)

Fig. 6. STK35 KO mice show ovary defects.

Ectopic expression of wt-DAO, but not the inactive mutant, promotes senescence. Ectopic expression of wt-DAO, but not the inactive mutant, promotes senescence.

Reb does not play a significant role in regulating TGF-β signaling

Volume 9, Pages (November 2018)

TOR, but not OSP, blocks STx2B transport and alters endosomal dynamics

SIRT1 is downregulated in human gastric cancer (GC).

Attenuated STM expression in drn and drnl mutants.

FACT facilitates the deposition of H2A–H2B onto a hexasome.

Expression of PLT1 mRNA during embryogenesis and the effects of PLT1 misexpression in the an3 han mutant. Expression of PLT1 mRNA during embryogenesis.

DRN and DRNL regulate STM expression via binding to a conserved promoter motif. DRN and DRNL regulate STM expression via binding to a conserved promoter.

(A) Western blot probing nuclear extract from wild-type (wt) and the newly generated ACF1 mutant (AcfC) embryos (0–16 h). (A) Western blot probing nuclear.

Shp2 is required for lens and lacrimal gland development.

Comparison of biological replicates for Ery-treated samples.

MiR-431-5p directly targets RAF1 and is negatively correlated to RAF1 expression (A) The predicted binding sites of miR-431-5p and RAF1 3′-UTR, and mutant.

FBXL19-AS1 acts as a sponge of miR-431-5p in lung cancer (A) The predicted binding sites of FBXL19-AS1 and miR-431-5p, and the mutant sites in mutant-type.

miR-9 regulation of Pax6 expression.

Gene Amplification as a Developmental Strategy

Fig. 1. DEL-1 is expressed by human and mouse osteoclasts.

Fig. 2 TGF-β1 signaling drives fibrosis in Neu1−/− muscle, which is rescued by AAV-mediated gene therapy. TGF-β1 signaling drives fibrosis in Neu1−/− muscle,

Subperineurial glia are required for normal GluRIIA distribution.

Wolbachia bacterial infection does not affect the expression of Imd pathway components and ROS-producing oxidases. Wolbachia bacterial infection does not.

CDDO-Me induces apoptosis independent of Bcl-2 level as well as p53 status in human NSCLC cells. CDDO-Me induces apoptosis independent of Bcl-2 level as.

Regulation of the SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE genes/microRNA156 Module by the Homeodomain Proteins PENNYWISE and POUND- FOOLISH in Arabidopsis

Fig. 1 Dcr-2 is required for Toll signaling in Drosophila S2 cells.

Mutations in the zebrafish hmgcs1 gene reveal a novel function for isoprenoids during red blood cell development by Jose A. Hernandez, Victoria L. Castro,

Volume 23, Issue 11, Pages (June 2018)

The bHLH Transcription Factors MYC2, MYC3, and MYC4 Are Required for Jasmonate- Mediated Inhibition of Flowering in Arabidopsis Houping Wang, Yang Li,

NRG2 Affects Nitrate Accumulation and Uptake

Fig. 2 Dcr-2 is required for Toll activation in adult flies.

Binding of TCP4-Like Proteins to the SAUR Promoters Is Enhanced by Light Signal or the Lack of PIFs. (A) and (B) Light increases the binding of TCP4 to.

Presentation transcript:

Loss of Chk2 DNA damage signaling does not restore embryogenesis in vas mutant flies. Loss of Chk2 DNA damage signaling does not restore embryogenesis in vas mutant flies. (A) Immunohistochemical detection of HeT-A/Gag protein in WT (w1118; a), vasD1/D1; nos-Gal4>GFP-vasWT (b and c), and vasD1/D1; vas-Gal4>GFP-vasWT (d) stage 5 embryos. Arrows indicate WT localization of HeT-A/Gag. Staining of the whole embryos is presented in Fig S3A. Scale bars indicate 10 and 5 μm (5× magnification). (B) qPCR analysis of LTR transposons burdock, blood, and gypsy and non-LTR transposon HeT-A RNAs in ovaries from vasD1/D1 single and vasD1/D1, mnkP6/P6 double mutant flies, and mnkP6/P6 and agot2/t3 mutant flies. The expression level of transposons in WT (w1118) was set to one and normalized to rp49 mRNA in individual experiments. Error bars represent the standard deviation among three biological replicates. Oogenesis completion is indicated with + and −. (C) qPCR analysis of LTR transposons burdock, blood, and gypsy and non-LTR transposon HeT-A RNAs in early embryos produced by vasD1/D1, mnkP6/P6 double mutant, and mnkP6/P6 and agot2/t3 single mutant flies. The expression level of transposons in WT (w1118) was set to one and normalized to 18S rRNA in individual experiments. Error bars represent the SD among three biological replicates. (D) Immunohistochemical detection of HeT-A/Gag protein in stage 5 embryos from vasD1/D1, mnkP6/P6 double mutant and agot2/t3 single mutant flies. Staining of the whole embryos is presented in Fig S4D. Scale bars indicate 10 and 5 μm (5× magnification). Zeljko Durdevic et al. LSA 2018;1:e201800179 © 2018 Ephrussi et al.