Evidence for Mechanosensitive Channel Activity of Tentonin 3/TMEM150C Gyu-Sang Hong, Byeongjun Lee, Uhtaek Oh  Neuron  Volume 94, Issue 2, Pages 271-273.e2 (April 2017) DOI: 10.1016/j.neuron.2017.03.038 Copyright © 2017 Terms and Conditions

Figure 1 Comparison and Computer Simulation of Piezo1 and TTN3 Currents and Changes in Anion/Cation Permeability Ratios of TTN3 Mutants (A–C) Representative traces of MA currents of HEK cells transfected with human Piezo1 (A), Ttn3 (B), and Piezo1+Ttn3 (C). After whole cells are formed, the cells were poked with a glass pipette to 6 μm for 600 ms. T1 and T2 represent the inactivation time constants that were fitted by a biexponential function. (D) A computer simulation of a numerical sum of the two traces shown in (A) and (B). (E) The alignment of amino acid sequences of TRPV1, TRPV2, TRPV4, human TTN3 (hTTN3), and mouse TTN3 (mTTN3). Bold letters represent the selectivity filters of TRPV channels. (F) Current-voltage curves obtained from each TTN3 wild-type (WT) and Ttn3 mutant during mechanical stimulation in F11 cells. The pipette and bath solution contained 70 and 210 mM NaCl, respectively. (G) Anion/cation permeability ratios (PCl/PNa) of WT and Ttn3 mutants. The PCl/PNa of WT or each mutant was calculated by the reversal potential using the Goldman-Hodgkin-Katz equation (n = 7–10). ∗∗∗p < 0.001 compared to WT, one-way ANOVA, Tukey’s post hoc test. Error bars represent SEM. Neuron 2017 94, 271-273.e2DOI: (10.1016/j.neuron.2017.03.038) Copyright © 2017 Terms and Conditions