Complementation of rpn11-14ts with overexpression of Ded1p helicase in TBSV recombination in yeast. Complementation of rpn11-14ts with overexpression of.

Slides:



Advertisements
Similar presentations
Temperature at the western boundary (T west ) required at each time-step Timestep 1: T west = 0 o C Timestep 2: T west = -1 o C Timestep 3: T west = -2.
Advertisements

Figure 1.1 The observer in the truck sees the ball move in a vertical path when thrown upward. (b) The Earth observer views the path of the ball as a parabola.
Chemistry 2100 Chapter 26. The Central Dogma The central dogma of molecular biology: –Information contained in DNA molecules is expressed in the structure.
Wu et al., Supplementary Fig 1 Supplementary Figure 1: The depletion of BRG1 in HeLa cells has no effect on cell proliferation. Sh- empty vector was used.
A B C MDA-MB-231 OHT: h FOXM1 PLK CDC25b ERα Tubulin
Vegetal RNAs antagonise the dorsalising and BMP-inhibiting activity of Vrtn. Vegetal RNAs antagonise the dorsalising and BMP-inhibiting activity of Vrtn.
HI !.
Expression of adenylyl cyclases and miR‐142‐3p in CD4+CD25− T cells and CD4+CD25+ regulatory T cells. Expression of adenylyl cyclases and miR‐142‐3p in.
Dominant inhibitory effect of PG on the in vitro RdRp activity of the p92-Δ167N RdRp protein. Dominant inhibitory effect of PG on the in vitro RdRp activity.
1,25‐dihydroxyvitamin D3 and retinoic acid induce p19ink4d expression.
PDE5A is expressed by podocytes.
c‐Abl acetylation on Lys 730 promotes myogenic differentiation.
Defining the sequence within the TBSV p33 protein needed for binding to the Rsp5p WW-domain protein in vitro. Defining the sequence within the TBSV p33.
NM1 localizes in nucleoli and is required for Pol I transcription.
Themis acts to increase phosphorylation of Shp1.
RNA charging levels before and after starvation.
Rpn11p is recruited to the tombusvirus replicase in yeast.
Reduced copurification of selected co-opted host proteins with the viral replicase from yeast coexpressing WW-domain protein. Reduced copurification of.
In vitro activation of the RdRp function of the N-terminally truncated p92pol replication protein depends on cellular Hsp70 chaperone. In vitro activation.
Representative Examples of NGF and SK2 Protein Expression Within LSG A and B show representative Western blot images of NGF and SK2; C and D show corresponding.
Luminex phosphorylation measurements are reproducible and have broad dynamic range Individual difference in insulin‐induced phosphorylation of ERK measured.
Hannah M. Jaag, Judit Pogany, Peter D. Nagy  Cell Host & Microbe 
The MSP domain of MOSPD2 is sufficient to interact with STARD3 and STARD3NL The MSP domain of MOSPD2 is sufficient to interact with STARD3 and STARD3NL.
A B CDK2 CDK2 inhibition P Activated KRAS P CP110 CP110
John T. Arigo, Kristina L. Carroll, Jessica M. Ames, Jeffry L. Corden 
Western blot of Aβ42-specific antibody compared with 4G8 antibody.
Efficient copurification of selected co-opted host proteins with the viral replicase from yeast coexpressing TPR-domain protein or cyclophilin A (CypA).
Tomato bushy stunt virus Co-Opts the RNA-Binding Function of a Host Metabolic Enzyme for Viral Genomic RNA Synthesis  Robert Yung-Liang Wang, Peter D.
Effects of phospholipids on the in vitro RNA-binding activity of the TBSV p33C replication protein. Effects of phospholipids on the in vitro RNA-binding.
The Glycolytic Pyruvate Kinase Is Recruited Directly into the Viral Replicase Complex to Generate ATP for RNA Synthesis  Chingkai Chuang, K. Reddisiva.
Effects of cell cycle and peroxisomal division mutant yeasts on TBSV RNA recombination. Effects of cell cycle and peroxisomal division mutant yeasts on.
Rpn11 overexpression inhibits TBSV recombination in plants.
Knockdown of Rpn11 inhibits TBSV accumulation in plants.
The proteasomal Rpn11 metalloprotease is a suppressor of TBSV RNA recombination in yeast. The proteasomal Rpn11 metalloprotease is a suppressor of TBSV.
The PbGAPDH G6 fragment interacts with recombinant CD68.
Rpn11p mutation affects the recruitment of some other host factors into the tombusvirus replicase in yeast. Rpn11p mutation affects the recruitment of.
Models showing the proposed function of subverted cellular Rpn11p metalloprotease in TBSV replication and viral RNA recombination. Models showing the proposed.
Models for the role of WW-domain proteins in regulation of tombusvirus replication. Models for the role of WW-domain proteins in regulation of tombusvirus.
Limited transcriptomic changes upon HOTAIR RNA overexpression in MDA‐MB‐231 breast cancer cells Limited transcriptomic changes upon HOTAIR RNA overexpression.
Effect of phospholipids on the in vitro RdRp activity of the p92-Δ167N RdRp protein. Effect of phospholipids on the in vitro RdRp activity of the p92-Δ167N.
Partial recovery of TBSV RNA replication in yeast overexpressing selected subverted proviral host proteins. Partial recovery of TBSV RNA replication in.
Overexpression of BTAK/Aurora-A in ovarian carcinoma.
Comparable effects of phospholipids on the in vitro RNA-binding activities of the CIRV p95-Δ200N and the TBSV p92-Δ167N RdRp proteins. Comparable effects.
Dnmt2 mutants show delayed immune responses and Dnmt2–EGFP relocalizes and interacts with DCV RNA during infection. Dnmt2 mutants show delayed immune responses.
Fig. 4. Expression of p75NTR and time-course of TrkA autophosphorylation at the Y751 and Y490 sites in PC12-27 cells transfected with the vector, empty.
Tomoregulin-1 inhibits the TGFβ non-canonical (TAK1-JNK) pathways after pressure overload. Tomoregulin-1 inhibits the TGFβ non-canonical (TAK1-JNK) pathways.
Western blot analysis of histone H1.X.
RECQL4 is a RanGTP dependent MAP
MiR-200c/141 overexpression in LS-8 cells induces E-cad expression and cell-cell adhesion. miR-200c/141 overexpression in LS-8 cells induces E-cad expression.
Knockdown of endogenous WT-hTER by a short hairpin RNA (siRNA) directed specifically against the human WT telomerase RNA template. Knockdown of endogenous.
IL-6 inhibits insulin-induced formation of p85/IRS-1 complexes.
Elevation of protein levels of O-GlcNAcase in pre-diabetic and diabetic erythrocytes. Elevation of protein levels of O-GlcNAcase in pre-diabetic and diabetic.
MiR-431-5p directly targets RAF1 and is negatively correlated to RAF1 expression (A) The predicted binding sites of miR-431-5p and RAF1 3′-UTR, and mutant.
Increased proIGF-II and decreased IGF-IIR in BTBR compared with B6 mice. Increased proIGF-II and decreased IGF-IIR in BTBR compared with B6 mice. Western.
Distribution of specific proteins of various cell fractions and standard calibration curves of Cpd-1. Distribution of specific proteins of various cell.
GOLPH3 interacts with COG complex proteins.
A statistic for microtubule order in mitotic spindles.
For More Details:
WW domain does not show dominant-negative effect on TBSV replication in a yeast cell extract. WW domain does not show dominant-negative effect on TBSV.
The trimeric coiled‐coil HSBP1 protein promotes WASH complex assembly at centrosomes The stable MDA‐MB‐231 cell line expressing FLAG‐HaloTag‐WASH (FHT‐WASH)
Expression of CRC stem cell markers and L1 in CRC cells.
WW domain of Rsp5p blocks TBSV RNA replication in yeast.
Met is expressed in Her2-overexpressing cell lines and Her2 (+) breast tumors. Met is expressed in Her2-overexpressing cell lines and Her2 (+) breast tumors.
Interaction with PRMT5 involves the RBD1,2 and RGG domains of nucleolin. Interaction with PRMT5 involves the RBD1,2 and RGG domains of nucleolin. A, schematic.
Fig. 4 Dcr-2 binds to the 3′UTR of Toll mRNA.
HOXA11-AS acts as a ceRNA for miR-1297.
Characterization of SFV-specific small RNAs bound by Piwi4 and Ago2 in Aag2 cells. Characterization of SFV-specific small RNAs bound by Piwi4 and Ago2.
KDM4A levels affect the distribution of translation initiation factors
Fig. 2 Dcr-2 is required for Toll activation in adult flies.
Fig. 2 hTERT induces expression of heat shock protein genes through HSF1 and interacts with Hsp70-1. hTERT induces expression of heat shock protein genes.
Presentation transcript:

Complementation of rpn11-14ts with overexpression of Ded1p helicase in TBSV recombination in yeast. Complementation of rpn11-14ts with overexpression of Ded1p helicase in TBSV recombination in yeast. The effect of overexpression of the yeast 6×His-tagged Ded1p helicase on TBSV RNA recombination in rpn11-14ts yeasts at permissive temperature is shown. The RNA template was the recombinogenic DI-AU-FP RNA. See further details on Northern and Western blotting in the legend to Fig. 1. K. Reddisiva Prasanth et al. J. Virol. 2015;89:2750-2763