Volume 38, Issue 4, Pages (May 2010)

Slides:



Advertisements
Similar presentations
Volume 56, Issue 3, Pages (November 2014)
Advertisements

SAICAR Induces Protein Kinase Activity of PKM2 that Is Necessary for Sustained Proliferative Signaling of Cancer Cells  Kirstie E. Keller, Zainab M. Doctor,
Jaya Sahni, Andrew M. Scharenberg  Cell Metabolism 
Volume 60, Issue 4, Pages (November 2015)
Volume 33, Issue 2, Pages (January 2009)
Volume 67, Issue 3, Pages e4 (August 2017)
Volume 61, Issue 2, Pages (January 2016)
Volume 18, Issue 5, Pages (November 2013)
Volume 18, Issue 11, Pages (November 2011)
Volume 120, Issue 2, Pages (January 2005)
Sue Ann Krause, Joseph V. Gray  Current Biology 
Volume 23, Issue 14, Pages (July 2013)
Volume 44, Issue 3, Pages (November 2011)
A Metabolic (Re-)Balancing Act
Volume 11, Issue 6, Pages (June 2010)
Volume 51, Issue 2, Pages (July 2013)
Glutaminolysis and Transferrin Regulate Ferroptosis
Sue Ann Krause, Joseph V. Gray  Current Biology 
Volume 18, Issue 3, Pages (April 2005)
Christian M. Metallo, Matthew G. Vander Heiden  Molecular Cell 
Volume 7, Issue 3, Pages (May 2014)
Volume 137, Issue 5, Pages (May 2009)
Volume 21, Issue 4, Pages (February 2006)
mTOR Regulates Cellular Iron Homeostasis through Tristetraprolin
Volume 162, Issue 3, Pages (July 2015)
Volume 46, Issue 4, Pages (May 2012)
Bidirectional Transport of Amino Acids Regulates mTOR and Autophagy
Volume 41, Issue 2, Pages (January 2011)
Distinct Autophagosomal-Lysosomal Fusion Mechanism Revealed by Thapsigargin- Induced Autophagy Arrest  Ian G. Ganley, Pui-Mun Wong, Noor Gammoh, Xuejun.
Role of adiponectin in human skeletal muscle bioenergetics
Volume 91, Issue 5, Pages (November 1997)
Volume 12, Issue 1, Pages (July 2015)
Volume 29, Issue 5, Pages (March 2008)
TSC2 regulates VEGF through mTOR-dependent and -independent pathways
Volume 6, Issue 3, Pages (September 2000)
Volume 153, Issue 4, Pages (May 2013)
PRAS40 Is an Insulin-Regulated Inhibitor of the mTORC1 Protein Kinase
TNF-Induced Activation of the Nox1 NADPH Oxidase and Its Role in the Induction of Necrotic Cell Death  You-Sun Kim, Michael J. Morgan, Swati Choksi, Zheng-gang.
MTOR Inhibition Restores Amino Acid Balance in Cells Dependent on Catabolism of Extracellular Protein  Michel Nofal, Kevin Zhang, Seunghun Han, Joshua.
by Issam Ben-Sahra, Gerta Hoxhaj, Stéphane J. H. Ricoult, John M
Volume 62, Issue 3, Pages (May 2016)
Proteolytic Cleavage of Opa1 Stimulates Mitochondrial Inner Membrane Fusion and Couples Fusion to Oxidative Phosphorylation  Prashant Mishra, Valerio.
Volume 24, Issue 3, Pages (September 2016)
Volume 20, Issue 3, Pages (July 2017)
Glutaminolysis Activates Rag-mTORC1 Signaling
Biochemical Underpinnings of Immune Cell Metabolic Phenotypes
Volume 54, Issue 5, Pages (June 2014)
Volume 50, Issue 2, Pages (April 2013)
Volume 22, Issue 11, Pages (March 2018)
Volume 49, Issue 1, Pages (January 2013)
Volume 26, Issue 6, Pages (June 2007)
Attenuation of LDH-A expression uncovers a link between glycolysis, mitochondrial physiology, and tumor maintenance  Valeria R. Fantin, Julie St-Pierre,
Volume 20, Issue 2, Pages (February 2012)
Volume 47, Issue 6, Pages (September 2012)
Volume 33, Issue 5, Pages (March 2009)
Volume 67, Issue 6, Pages (June 2005)
Volume 52, Issue 2, Pages (October 2013)
Volume 24, Issue 19, Pages (October 2014)
Prolonged Rapamycin Treatment Inhibits mTORC2 Assembly and Akt/PKB
Teemu P. Miettinen, Mikael Björklund  Cell Reports 
Volume 67, Issue 3, Pages e4 (August 2017)
Volume 26, Issue 11, Pages e4 (March 2019)
Volume 38, Issue 1, Pages (April 2010)
Vidhya Ramachandran, Khyati H. Shah, Paul K. Herman  Molecular Cell 
Volume 49, Issue 1, Pages (January 2013)
MTOR Inhibition Restores Amino Acid Balance in Cells Dependent on Catabolism of Extracellular Protein  Michel Nofal, Kevin Zhang, Seunghun Han, Joshua.
Volume 57, Issue 2, Pages (January 2015)
Volume 11, Issue 5, Pages (May 2010)
Volume 58, Issue 3, Pages (May 2015)
Nitrogen Regulates AMPK to Control TORC1 Signaling
Presentation transcript:

Volume 38, Issue 4, Pages 487-499 (May 2010) Glucose Addiction of TSC Null Cells Is Caused by Failed mTORC1-Dependent Balancing of Metabolic Demand with Supply  Andrew Y. Choo, Sang Gyun Kim, Matthew G. Vander Heiden, Sarah J. Mahoney, Hieu Vu, Sang-Oh Yoon, Lewis C. Cantley, John Blenis  Molecular Cell  Volume 38, Issue 4, Pages 487-499 (May 2010) DOI: 10.1016/j.molcel.2010.05.007 Copyright © 2010 Elsevier Inc. Terms and Conditions

Figure 1 mTORC1 Suppression Protects TSC-Deficient Cells from Glucose Deprivation-Induced Death through a p53-Independent Mechanism (A) TSC1+/+ p53+/+ and TSC1−/− p53+/+ MEFs were deprived of glucose for 48 hr with or without rapamycin, and phase microscopy was used to observe cell viability. (B) Cell viability from (A) was measured via propidium iodide (PI) exclusion assay. (C) ELT-3 and LExF were deprived of glucose for 72 hr with or without rapamycin. (D) Phase image of TSC2−/− p53−/− or TSC2+/+ p53−/− MEFs deprived of glucose for 60 hr. (E) Western blot of p53. (F) PI exclusion assay from (D). (G) Cell viability following raptor knockdown. (H) Knockdown efficiency for raptor and phospho-S6K1. Shown is an average (+SEM) of three independent experiments. All data (mean ±SEM) are representative of three independent experiments. Molecular Cell 2010 38, 487-499DOI: (10.1016/j.molcel.2010.05.007) Copyright © 2010 Elsevier Inc. Terms and Conditions

Figure 2 mTORC1 Inhibition Maintains Cellular Bioenergetics in the Absence of Glucose (A) The cellular morphology of TSC2−/− p53−/− control and Bcl-XL-expressing cells after 48 hr of glucose withdrawal. (B) The expression level of Bcl-XL and the phosphorylation of S6K1. (C) Cell viability of TSC2−/− p53−/− Bcl-XL cells at 96 hr after serum withdrawal. Shown is an average (±SEM) of three independent experiments. (D) The viability of control or Bcl-XL-expressing TSC2−/− p53−/− MEFs at 60 hr of glucose deprivation. Shown is an average (+SEM) of three independent experiments. (E) Cell size of TSC2−/− p53−/− Bcl-XL MEFs. (F) F- versus G-actin population after glucose deprivation in TSC2−/− p53−/− Bcl-XL MEFs. JASP, Jasplakinolide (1 μM); LatA, Latrunculin A (2 μM). (G) Cellular ATP levels in control or Bcl-XL-expressing MEFs were determined after 24 hr of glucose deprivation with or without rapamycin. The control cells were plated at the same density and given fresh media at the time of glucose deprivation. Shown is an average (±SEM) of three independent experiments. (H) ATP levels were measured following 24 hr of glucose deprivation in TSC2+/+ and TSC2−/− cells. (I) ATP and ADP levels in the same conditions as (H). ATP, p < 0.0001; ADP, p = 0.11. (J) ATP/ADP ratio was determined from (H). (K) AMPKα phosphorylation (T172) and ACC phosphorylation were measured 24 hr after glucose withdrawal. Molecular Cell 2010 38, 487-499DOI: (10.1016/j.molcel.2010.05.007) Copyright © 2010 Elsevier Inc. Terms and Conditions

Figure 3 L-Glutamine Is Required for Rapamycin-Mediated ATP Maintenance in the Absence of Glucose (A) TSC2−/− p53−/− MEFs were deprived of glucose, amino acids, or both with or without rapamycin. (B) Cell viability of cell with branched-chain amino acids (BCAAs), L-glutamine, or total amino depletion. (C) Total cellular ATP levels were determined after 24 hr of glucose deprivation. The ATP levels are an average (±SEM) of three independent experiments. (D) S6K1 (T389) phosphorylation after 24 hr of deprivation. (E) L-glutamine was added to glucose and total amino acid-deprived conditions at the time of deprivation, and viability was measured at 60 hr after withdrawal. (F) A time course measuring the viability of TSC2−/− p53−/− MEFs grown in complete, (−)glucose, (−)Glu (−)aa, and (−)Glu (−)aa plus 4 mM L-glutamine media. The samples labeled with X were replenished with only glutamine (2 mM) starting at 48 hr and replenished every 24 hr. The Rap X sample also got 20 nM of rapamycin at 0 and 72 hr. (G) Phase images were taken at 3 days and 6 days with or without L-glutamine (2 mM) retreatment in (−)glucose (−)aa conditions. Shown is an average (+SEM) of three independent experiments. All viability experiments are representative of at least three independent experiments. Molecular Cell 2010 38, 487-499DOI: (10.1016/j.molcel.2010.05.007) Copyright © 2010 Elsevier Inc. Terms and Conditions

Figure 4 Mitochondrial Activity Is Not Increased in Rapamycin-Treated TSC2−/− p53−/− MEFs (A) TSC2−/− p53−/− MEFs were deprived of glucose for 24 hr, and the rate of oxygen consumption was measured. Oxygen consumption was measured by Clark's electrode, and the rate of consumption was determined and indicated as nmol of oxygen per minute per million cells. The experiment was conducted three times and yielded similar results. (B) Mitochondrial membrane potential was measured at 12 and 24 hr after treatment conditions via DiOC6 MFI via FACS. Oligomycin was used as a positive control (5 μg/ml) in cells grown in glucose containing media (n = 3) (±SEM) of three independent experiments. (C) PGC-1α and cytochrome c protein levels were determined at 24 hr postdeprivation. (D) MitoTracker measurement in the absence of glucose and with or without rapamycin. Molecular Cell 2010 38, 487-499DOI: (10.1016/j.molcel.2010.05.007) Copyright © 2010 Elsevier Inc. Terms and Conditions

Figure 5 mTORC1 Regulates Metabolic Consumption, and Modulating Consumption Is Necessary and Sufficient to Regulate Survival in the Absence of Glucose (A) TSC2−/− p53−/− MEFs were incubated with rapamycin for 24 hr, cycloheximide (CHX) (5 μg/ml) for 12 hr, and amino acid-deprived media with glutamine, (−)aa (+)Gln, for 12 hr. Thereafter, the cells were briefly washed in (−)Glu (−)aa media and incubated with glucose-free media containing oligomycin (10 μg/ml) and antimycin A (2 μg/ml). The (−)aa (+)Gln samples were incubated in (−)Glu, (−)aa, (+)Gln media with oligomycin and antimycin. The cells were immediately incubated in 37°C, and ATP levels were measured at indicated time points. All media used for washing and incubating were maintained at 37°C at all times. Experiments were carried out in pentuplicate, and data are presented as a percent of control, which is the ATP level prior to incubation in the glucose-free/oligomycin/antimycin media. Student's t test: ∗p < 0.001 compared to control. (B) ATP levels were measured 24 hr after glucose deprivation in TSC2−/− p53−/− MEFs incubated with rapamycin (20 nM), ouabain (1 mM), and rapamycin/gram D (1 μg/ml). GramD, gramicidin D. Student's t test: (−) Glu compared to (−)Glu + Ouabain = p < 0.01. (C) Cell viability was measured 60 hr after glucose deprivation. (D) Cell viability for the effect of gram D at the indicated concentrations was measured at 60 hr after glucose deprivation. (E) ATP levels were measured similarly to (A), except CHX (5 μg/ml) was used. Gramicidin D (2 μg/ml) was given to both samples 5 hr prior to lysis. This amount was used because we observed that a higher concentration was necessary to have a much more dramatic effect on CHX-treated cells. (F) Cell viability of the groups was measured at indicated time points with PI exclusion assay. (G) The activation status of S6K1 (T389) was measured from cells deprived of glucose for 24 hr and treated with rapamycin, ouabain (1 mM), and CHX (5 μg/ml). Shown is an average (+SEM) of three independent experiments for all viability experiments. All viability experiments are an average (±SEM) of three independent experiments. Molecular Cell 2010 38, 487-499DOI: (10.1016/j.molcel.2010.05.007) Copyright © 2010 Elsevier Inc. Terms and Conditions

Figure 6 TCA Cycle Intermediates Can Substitute for Glutamine Only When Energetic Consumption Is Decreased (A) A diagram showing the TCA cycle intermediates, and the compounds used for this study are indicated with asterisks. (B) TSC2−/− p53−/− MEFs were deprived of glucose and given an extra 4 mM or 8 mM of glutamine to give final concentrations of 4 mM, 8 mM, and 12 mM. Cell viability was measured at 60 hr after deprivation. (C) TSC2−/− p53−/− MEFs were deprived of amino acids and glucose and given the indicated molecules. Cell viability was measured at 48 hr after deprivation. Shown is an average (+SEM) of three independent experiments. All data are an average (±SEM) of three independent experiments. Molecular Cell 2010 38, 487-499DOI: (10.1016/j.molcel.2010.05.007) Copyright © 2010 Elsevier Inc. Terms and Conditions

Figure 7 Pharmacologic Inhibition of the Glutamine Metabolism Pathway Induces Cytotoxicity under Glucose-Limiting Conditions (A) A diagram showing the enzymes involved in glutamate metabolism (see text for more details). (B) EGCG (50 μM) and AOA (0.25–5.0 mM) were tested for cytotoxicity of TSC2−/− cells deprived of glucose and given rapamycin (48 hr after deprivation). Where indicated, various metabolites α-ketoglutarate (10 mM), glutamate (4 mM), and pyruvate (1 mM) were also supplemented. (C) TSC2−/− cells were infected with shRNA constructs (#1–4) targeting GDH, and viability after 60 hr without glucose and with or without rapamycin is shown. (D) TSC2−/− cells deprived of glucose and amino acids, but supplemented with glutamine (4 mM), were given EGCG (50 μM) or AOA (2 mM). All data are an average (±SEM) of three independent experiments. Molecular Cell 2010 38, 487-499DOI: (10.1016/j.molcel.2010.05.007) Copyright © 2010 Elsevier Inc. Terms and Conditions