High resolution melting (HRM) of forensically informative SNPs

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High resolution melting (HRM) of forensically informative SNPs Bhavik Mehta, Runa Daniel, Dennis McNevin  Forensic Science International: Genetics Supplement Series  Volume 4, Issue 1, Pages e376-e377 (January 2013) DOI: 10.1016/j.fsigss.2013.10.191 Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Principle of HRM (adapted from http://www.appliedbiosystems.com/HRM products). Forensic Science International: Genetics Supplement Series 2013 4, e376-e377DOI: (10.1016/j.fsigss.2013.10.191) Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 Difference plots for SNP rs12913832 (C/T) using both the custom designed HRM primers and IrisPlex published primer sets showing three genotype variants (CC, CT and TT) over 10ng (A) and 1ng (B) DNA template amounts for eight samples. Forensic Science International: Genetics Supplement Series 2013 4, e376-e377DOI: (10.1016/j.fsigss.2013.10.191) Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions