Sol i 1, the phospholipase allergen of imported fire ant venom

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Presentation transcript:

Sol i 1, the phospholipase allergen of imported fire ant venom Donald R. Hoffman, PhD, Rhonda H. Sakell, BS, Margit Schmidt, PhD Journal of Allergy and Clinical Immunology Volume 115, Issue 3, Pages 611-616 (March 2005) DOI: 10.1016/j.jaci.2004.11.020 Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 1 cDNA sequence and translation for Sol i 1. The yellow and green shaded areas have been verified by amino acid sequencing of peptides. The green shaded regions determine the enzyme active site with the catalytic residues shown enlarged. Underlines indicate potential N-linked glycosylation. The end of the signal sequence is indicated by / and the propeptide cleavage by //. The termination codon is indicated by ∗. Journal of Allergy and Clinical Immunology 2005 115, 611-616DOI: (10.1016/j.jaci.2004.11.020) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 2 Sequence alignment of Sol i 1 with vespid venom phospholipase allergens. Strictly conserved residues are indicated by ∗, highly conserved by :, and partially conserved by a . under the alignments. A – indicates an insertion to maximize alignment. Journal of Allergy and Clinical Immunology 2005 115, 611-616DOI: (10.1016/j.jaci.2004.11.020) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 3 Rooted phylogenetic tree for the amino acid sequences of the 9 phospholipases shown in Fig 2. Horizontal line distances are proportional to calculated phylogenetic differences. PL, Phospholipase. Journal of Allergy and Clinical Immunology 2005 115, 611-616DOI: (10.1016/j.jaci.2004.11.020) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 4 Silver-stained SDS-PAGE gel showing the results of concanavalin A agarose absorption of Solenopsis invicta venom. Std is molecular weight standards with values indicated in kilodaltons, Con A is the protein recovered after α-methyl-D-mannoside elution from the column, Sup is the column flow-through that was not absorbed, and Venom is unfractionated S invicta venom proteins. The>marks the position of Sol i 1. Journal of Allergy and Clinical Immunology 2005 115, 611-616DOI: (10.1016/j.jaci.2004.11.020) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions