TLR9 deficiency promotes aberrant T cell and myeloid dendritic cell (DC) phenotype in imiquimod-induced autoimmunity. TLR9 deficiency promotes aberrant.

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TLR9 deficiency promotes aberrant T cell and myeloid dendritic cell (DC) phenotype in imiquimod-induced autoimmunity. TLR9 deficiency promotes aberrant T cell and myeloid dendritic cell (DC) phenotype in imiquimod-induced autoimmunity. WT and Tlr9-/- mice were treated with imiquimod for 3 weeks. (A) Splenocytes were isolated and stained for CD45, CD4 and CD44. (B) Splenocytes were isolated and stained for CD45, CD4 and intracellularly stained for IFN-γ and IL-17A. (C) mRNA levels of IFN-γ in the spleen. (D) Splenocytes were isolated and stained for CD45, CD4 and intracellularly stained for GM-CSF and IL-10. (E) Splenocytes were isolated and stained for CD45, CD4 and intracellularly stained for FoxP3 (n=6/group). (F) Bone marrow-derived DCs (BMDC) from WT and Tlr9-/- mice were stimulated with medium (UN) and imiquimod (1 µg/mL) for 24 hours. TLR7 expression was determined by flow cytometry on CD11c+ DCs (n=4). GM-CSF, granulocyte-macrophage colony-stimulating factor; IFN, interferon; IL, interleukin; MFI, mean fluorescence intensity; TLR, toll-like receptor; WT, wild type; UN, unstimulated. Yudong Liu et al. Lupus Sci Med 2018;5:e000259 ©2018 by Lupus Foundation of America