Expression of mRNA and protein of macrophage colony-stimulating factor and its receptor in human follicular luteinized granulosa cells Ali Salmassi,

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Presentation transcript:

Expression of mRNA and protein of macrophage colony-stimulating factor and its receptor in human follicular luteinized granulosa cells Ali Salmassi, Ph.D., Zhifen Zhang, M.D., Andreas G. Schmutzler, M.D., Kerstin Koch, M.T.A., Sybille Buck, M.T.A., Walter Jonat, M.D., Liselotte Mettler, M.D. Fertility and Sterility Volume 83, Issue 2, Pages 419-425 (February 2005) DOI: 10.1016/j.fertnstert.2004.06.072 Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 Correlation (r = 0.677, P<.01, n = 85) between macrophage colony stimulating factor (M-CSF) levels in serum and in follicular fluid (FF) on the day of oocyte retrieval. The linear regression was M-CSF in FF = 10.2 × M-CSF in serum. Fertility and Sterility 2005 83, 419-425DOI: (10.1016/j.fertnstert.2004.06.072) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Immunocytochemical demonstration of macrophage colony-stimulating factor (M-CSF) and its receptor (M-CSFR). (A) M-CSF in the cytoplasma and also its localization on cell membrane surface of human follicular granulosa cells (GCs). (B) Localization of c-fms gene product (M-CSFR) on the surface of GCs. (C) GCs without the primary antibody as negative control. (D) Localization of M-CSFR on the surface of monocytes, isolated from blood as positive control. Immunoalkaline phosphatase reaction. Magnification, ×600. Fertility and Sterility 2005 83, 419-425DOI: (10.1016/j.fertnstert.2004.06.072) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 3 Effect of increasing culture time on macrophage colony-stimulating factor (M-CSF) secretion by granulosa cells (mean M-CSF levels, 95% confidence interval). Results from three experiments (five patients each) performed in triplicate (P<.05). Number of cells, 2 × 104 per well. Fertility and Sterility 2005 83, 419-425DOI: (10.1016/j.fertnstert.2004.06.072) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 4 In vitro reverse transcription (RT)–polymerase chain reaction (PCR) of macrophage colony-stimulating factor (M-CSF) and its receptor (M-CSFR). The lanes show the following: Lane a, the separation of a molecular weight marker; lanes b–d, M-CSF amplicon in human granulosa cells (GCs), in human monocytes, and in human placenta as positive control; lanes e–g, M-CSFR amplicon in human GCs, in human monocytes, and in human placenta as positive control; lanes h–j, glyceraldehye-3 phosphotase dehydrogenase (GAPDH) amplicon in human GCs, human monocytes, and placenta; lane k, negative control omitting RT. Fertility and Sterility 2005 83, 419-425DOI: (10.1016/j.fertnstert.2004.06.072) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions