Tolerance and immunity after sequential lung and bone marrow transplantation from an unrelated cadaveric donor Paul Szabolcs, MD, Rebecca H. Buckley,

Slides:

Advertisements

Similar presentations

Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy Erik Wambre, PhD, Jonathan.

Advertisements

Comparison of the allergic and nonallergic CD4+ T-cell responses to the major dog allergen Can f 1 Suvi Parviainen, MSc, Antti Taivainen, MD, PhD, Aino.

Triggering of specific Toll-like receptors and proinflammatory cytokines breaks allergen- specific T-cell tolerance in human tonsils and peripheral blood

Identification of drug- and drug-metabolite immune responses originating from both naive and memory T cells Andrew Gibson, MRes, Lee Faulkner, PhD, Sally.

Barbara Stanic, PhD, Willem van de Veen, PhD, Oliver F

Regulation of TH2 responses by the pulmonary Clara cell secretory 10-kd protein Chih-Hsing Hung, MD, Li-Chen Chen, MD, Zhongjian Zhang, PhD, Bhabadeb.

Therapeutic reversal of food allergen sensitivity by mature retinoic acid–differentiated dendritic cell induction of LAG3+CD49b−Foxp3− regulatory T cells

Flow cytometry imaging identifies rare TH2 cells expressing thymic stromal lymphopoietin receptor in a “proallergic” milieu Amanda J. Reefer, MS, Kathryn.

Potential role of extracellular vesicle–mediated antigen presentation in Helicobacter pylori hypersensitivity during eradication therapy Takamasa Ito,

Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy Yin Yao, MD, Cai-Ling.

Mechanical injury polarizes skin dendritic cells to elicit a TH2 response by inducing cutaneous thymic stromal lymphopoietin expression Michiko K. Oyoshi,

Ambient particulate matter directs nonclassic dendritic cell activation and a mixed TH1/TH2-like cytokine response by naive CD4+ T cells Marc A. Williams,

Cell-to-cell contact between activated CD4+ T lymphocytes and unprimed monocytes interferes with a TH1 response Miriam Wittmann, MD, Mareike Alter, Tanja.

IgE cross-linking impairs monocyte antiviral responses and inhibits influenza-driven TH1 differentiation Regina K. Rowe, MD, PhD, David M. Pyle, MD,

IL-25 and CD4+ TH2 cells enhance type 2 innate lymphoid cell–derived IL-13 production, which promotes IgE-mediated experimental food allergy Jee-Boong.

Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.

Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production Mark C. Glaum, MD, PhD, Shilpi Narula, MD, Decheng Song,

Peanut oral immunotherapy results in increased antigen-induced regulatory T-cell function and hypomethylation of forkhead box protein 3 (FOXP3) Aleena.

Distinct endotypes of steroid-resistant asthma characterized by IL-17Ahigh and IFN- γhigh immunophenotypes: Potential benefits of calcitriol Emma S. Chambers,

Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro Pierre Pochard, PhD, Brian Vickery, MD, M. Cecilia.

Reduction of CRKL expression in patients with partial DiGeorge syndrome is associated with impairment of T-cell functions Mauro Giacomelli, PhD, Rajesh.

IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs Chien-Chang Chen, PhD, Takao Kobayashi, PhD, Koji Iijima,

Malassezia sympodialis thioredoxin–specific T cells are highly cross-reactive to human thioredoxin in atopic dermatitis Hari Balaji, MSc, Annice Heratizadeh,

Long-term immune reconstitution after matched unrelated hematopoietic stem cell transplantation for immunodeficiency Vy Hong-Diep Kim, MD, Brenda Reid,

Type 3 innate lymphoid cells induce proliferation of CD94+ natural killer cells Shuo Li, PhD, Hideaki Morita, MD, PhD, Beate Rückert, Sci Tec, Tadech.

Induction and maintenance of allergen-specific FOXP3+ Treg cells in human tonsils as potential first-line organs of oral tolerance Oscar Palomares, PhD,

Eosinophils contribute to the resolution of lung-allergic responses following repeated allergen challenge Katsuyuki Takeda, MD, PhD, Yoshiki Shiraishi,

Triggering of specific Toll-like receptors and proinflammatory cytokines breaks allergen- specific T-cell tolerance in human tonsils and peripheral blood

Shared and restricted T-cell receptor use is crucial for carbamazepine-induced Stevens- Johnson syndrome Tai-Ming Ko, MS, Wen-Hung Chung, MD, PhD, Chun-Yu.

Tolerance-like mediated suppression by mesenchymal stem cells in patients with dust mite allergy–induced asthma Simi Kapoor, MD, Shyam A. Patel, PhD,

Clara cell 16-kd protein downregulates TH2 differentiation of human naive neonatal T cells Sofi Johansson, MSc, Göran Wennergren, MD, PhD, Nils Åberg,

Kathleen R. Bartemes, BA, Gail M. Kephart, BS, Stephanie J

T Cell Repertoire Development in XSCID Dogs Following Nonconditioned Allogeneic Bone Marrow Transplantation William Vernau, Brian J. Hartnett, Douglas.

Thymic stromal lymphopoietin converts human epidermal Langerhans cells into antigen- presenting cells that induce proallergic T cells Susanne Ebner, PhD,

Targeting Fel d 1 to FcγRI induces a novel variation of the TH2 response in subjects with cat allergy Kathryn E. Hulse, BS, Amanda J. Reefer, MS, Victor.

Augmentation of antitumor immune responses after adoptive transfer of bone marrow derived from donors immunized with tumor lysate-pulsed dendritic cells

Inhibition of human B-cell development into plasmablasts by histone deacetylase inhibitor valproic acid Anne-Kathrin Kienzler, MSc, Marta Rizzi, MD,

Victoria M. Leb, MSc, Beatrice Jahn-Schmid, PhD, Klaus G

Tolerogenic signaling by pulmonary CD1c+ dendritic cells induces regulatory T cells in patients with chronic obstructive pulmonary disease by IL-27/IL-10/inducible.

Patients with atopic dermatitis and history of eczema herpeticum elicit herpes simplex virus–specific type 2 immune responses Stephan Traidl, Petra Kienlin,

Allison K. Martin, BS, Douglas G. Mack, PhD, Michael T

Chronic cat allergen exposure induces a TH2 cell–dependent IgG4 response related to low sensitization Amedee Renand, PhD, Luis D. Archila, MSc, John.

Role of B cells in TH cell responses in a mouse model of asthma

Decreases in human dendritic cell–dependent TH2-like responses after acute in vivo IgE neutralization John T. Schroeder, PhD, Anja P. Bieneman, BS, Kristin.

Identification of drug- and drug-metabolite immune responses originating from both naive and memory T cells Andrew Gibson, MRes, Lee Faulkner, PhD, Sally.

CD25 expression distinguishes functionally distinct alloreactive CD4+ CD134+ (OX40+) T-cell subsets in acute graft-versus-host disease Philip R Streeter,

Increased frequency of dual-positive TH2/TH17 cells in bronchoalveolar lavage fluid characterizes a population of patients with severe asthma Chaoyu.

Peanut T-cell epitope discovery: Ara h 1

Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy Erik Wambre, PhD, Jonathan.

Sabah El-Ghaiesh, MSc, Joseph P

In vivo maturation of TH cells in relation to atopy

Staphylococcal enterotoxin A–activated regulatory T cells promote allergen-specific TH2 response to intratracheal allergen inoculation Wei-ping Zeng,

Direct ex vivo analysis of allergen-specific CD4+ T cells

Allergen-specific CD8+ T cells in peanut-allergic individuals

Amplification of Toll-like receptor–mediated signaling through spleen tyrosine kinase in human B-cell activation Shigeru Iwata, MD, PhD, Kunihiro Yamaoka,

Sphingosine-1-phosphate/sphingosine-1-phosphate receptor 1 signaling is required for migration of naive human T cells from the thymus to the periphery

Antigen-specific and nonspecific determinants of cytokine production during topical sensitization of mice to chemical allergens Artin Moussavi, PhDa,

Matthew J. Loza, PhD, Susan Foster, PhD, Stephen P

Harald Renz, MD, Chaya Brodie, PhD, Katherine Bradley, BS, Donald Y. M

Impairment of T-regulatory cells in cord blood of atopic mothers

In vivo effects of glucocorticoids on IgE production

Wasp venom immunotherapy expands a subpopulation of CD4+CD25+ forkhead box protein 3–positive regulatory T cells expressing the T-cell receptor Vβ2 and.

Pia J. Hauk, MDa, Qutayba A. Hamid, MD, PhDc, George P

CD25 deficiency causes an immune dysregulation, polyendocrinopathy, enteropathy, X- linked–like syndrome, and defective IL-10 expression from CD4 lymphocytes

IL-10–producing monocytes differentiate to alternatively activated macrophages and are increased in atopic patients Antje Prasse, MD, Martin Germann,

IgG4 production is confined to human IL-10–producing regulatory B cells that suppress antigen-specific immune responses Willem van de Veen, MSc, Barbara.

Natural history of cow’s milk allergy

CD137 Expression Identifies Leukemia Specific CTL after in Vitro Priming of Cord Blood T Cells Previously Expanded By CD3/CD28 Co-Stimulation Jeyaraj.

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites

Comparison of the allergic and nonallergic CD4+ T-cell responses to the major dog allergen Can f 1 Suvi Parviainen, MSc, Antti Taivainen, MD, PhD, Aino.

Presentation transcript:

Tolerance and immunity after sequential lung and bone marrow transplantation from an unrelated cadaveric donor Paul Szabolcs, MD, Rebecca H. Buckley, MD, Robert Duane Davis, MD, Jerelyn Moffet, PA, Judith Voynow, MD, Jeyaraj Antony, PhD, Xiaohua Chen, PhD, Gregory D. Sempowski, PhD, David W. Zaas, MD Journal of Allergy and Clinical Immunology Volume 135, Issue 2, Pages 567-570.e3 (February 2015) DOI: 10.1016/j.jaci.2014.07.058 Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 A, T-cell immunity as assessed by mitogen responses on a chronological scale. The y-axis shows counts per minute (CPM) following3 H-thymidine incorporation and measured as previously described.6 PBMCs were stimulated with PHA, pokeweed mitogen (PWM), concanavalin A (ConA), or medium alone as negative control. B, Lymphocyte reconstitution. Absolute numbers of lymphocyte subsets per μL/mm3 were measured as previously described7 from whole blood. C, Tempo of thymopoiesis is depicted by the absolute number of CD4+ T cells over time that display the combinatorial markers of “naive” T-cell immunophenotype (CD45RA/CD62L coexpression). D, Pulmonary function tests before and after BOLT. FEV1 and forced expiratory flow rates at 25% to 75% of FVC (FEF25-75) as measured by standard spirometry. Journal of Allergy and Clinical Immunology 2015 135, 567-570.e3DOI: (10.1016/j.jaci.2014.07.058) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Proliferative responses and cytokine secretion of donor T cells. Negatively selected (Easysep, Stem Cell Technologies, Vancouver, Calif) >95% pure CD3+ T cells (1 × 10−5 cells/well) isolated from the recipient (100% donor) 1 year after BMT (in vivo tacrolimus levels ∼3-4 ng/dL range) were cocultured for 5 days with 1 × 10−4 cells/well of EBV/LCL from the indicated sources. To analyze restriction elements in alloreactivity, EBV/LCLs were opsonized at 2 μg/mL with MHC class-I or class-II–blocking antibodies (clones W632 and L243, Bio-Legend Leaf, San Diego, Calif) before coculture. The impact of regulatory T-cell removal was assayed by depleting CD25+ cells among responders with IL-2 immunotoxin as previously described.7 Lung/marrow donor-derived EBV/LCL was not available to be tested as antigen-presenting cells. Culture supernatant was collected and cryopreserved for batched cytokine analysis by luminex assay on a Bio-Plex 200 system (Bio-Rad, Hercules, Calif) (see Table E2). Immediately thereafter, T-cell proliferation was quantitated (counts per minute) by3 H-thymidine incorporation7 and depicted on the y-axis. Significance was tested as indicated by paired 2-tailed Student t test. Journal of Allergy and Clinical Immunology 2015 135, 567-570.e3DOI: (10.1016/j.jaci.2014.07.058) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 A and B, T-cell receptor (TCR) Vβ repertoire. Before BMT there was a severely restricted and oligoclonal pattern (A) that is in stark contrast to the polyclonal TCR diversity displayed 18 months post-BMT shown on panel B reflecting robust thymopoiesis. The single monoclonal peak of Jurkat cell line in the Vβ8 family serves as internal control. TCRVβ spectratyping was performed as previously published.8,9 Journal of Allergy and Clinical Immunology 2015 135, 567-570.e3DOI: (10.1016/j.jaci.2014.07.058) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions