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Nat. Rev. Cardiol. doi:10.1038/nrcardio.2016.139 Figure 4 Use of genome editing to rapidly generate knockout and knock‑in mice Figure 4 | Use of genome editing to rapidly generate knockout and knock-in mice. The components of CRISPR/Cas9, with or without a DNA repair template, are injected into mouse single-cell embryos, whereupon they can introduce mutations at the target site in the genome. The embryos are implanted into surrogate mothers, and pups are screened for the desired knockout or knock-in mutations after birth. The process can be completed in 3 weeks, the duration of gestation for mice. The same approach has proven effective in a variety of species. Strong, A. & Musunuru, K. (2016) Genome editing in cardiovascular diseases Nat. Rev. Cardiol. doi:10.1038/nrcardio.2016.139