MiRNA Profiling Identifies Candidate miRNAs for Bladder Cancer Diagnosis and Clinical Outcome Nadine Ratert, Hellmuth-Alexander Meyer, Monika Jung, Poline.

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miRNA Profiling Identifies Candidate miRNAs for Bladder Cancer Diagnosis and Clinical Outcome Nadine Ratert, Hellmuth-Alexander Meyer, Monika Jung, Poline Lioudmer, Hans-Joachim Mollenkopf, Ina Wagner, Kurt Miller, Ergin Kilic, Andreas Erbersdobler, Steffen Weikert, Klaus Jung The Journal of Molecular Diagnostics Volume 15, Issue 5, Pages 695-705 (September 2013) DOI: 10.1016/j.jmoldx.2013.05.008 Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 The miRNA expression in 57 tissue samples from nonmalignant and malignant bladder cancer. The expression of seven up-regulated miRNAs (A) and eight down-regulated (B) miRNAs is shown. The white columns are the nonmalignant tissues (n = 17). The light gray columns are the NMIBCs (n = 25). The dark gray columns are the MIBCs (n = 15). These columns depict medians with 95% confidence intervals determined by bootstrapping with 2000 re-samples. For the sake of completeness, we also include the deregulated miRNA data of miR-20a and miR-200a, which was previously reported.21 The expression was normalized against the reference miRNA combination of miR-101, miR-148b, miR-125a-5p, and miR-151-5p.21 For all miRNAs, the Kruskal-Wallis test was always statistically significant (P < 0.0001). Statistical differences between the three groups were pairwise calculated by the U-test and are indicated by asterisks. ∗P < 0.0167, ∗∗P < 0.0033, and ∗∗∗P < 0.00033 based on the Bonferroni correction for multiple testing. The Journal of Molecular Diagnostics 2013 15, 695-705DOI: (10.1016/j.jmoldx.2013.05.008) Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 2 Association of expression levels of miR-141 and miR-205 in malignant tissue samples, as determined by real-time RT-qPCR with overall survival time. Kaplan-Meier analysis correlating overall survival time as a function of miR-141 (A; log-rank test; P = 0.02) or miR-205 (B; log-rank test; P = 0.04) expression in tumor samples (n = 40). The overall survival time is defined as months elapsed between transurethral resection or radical cystectomy and death or the last follow-up date. Statistical significance is defined as P < 0.05. RTX, radical cystectomy; TURB, transurethral resection. The Journal of Molecular Diagnostics 2013 15, 695-705DOI: (10.1016/j.jmoldx.2013.05.008) Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions