Epidermal Stem Cells in the Isthmus/Infundibulum Influence Hair Shaft Differentiation: Evidence from Targeted DLX3 Deletion Jin-Chul Kim, Olivier Duverger,

Slides:

Advertisements

Similar presentations

Cyclic Alopecia and Abnormal Epidermal Cornification in Zdhhc13-Deficient Mice Reveal the Importance of Palmitoylation in Hair and Skin Differentiation

Advertisements

Ceramide Synthase 4 Regulates Stem Cell Homeostasis and Hair Follicle Cycling Franziska Peters, Susanne Vorhagen, Susanne Brodesser, Kristin Jakobshagen,

Progressive Alopecia Reveals Decreasing Stem Cell Activation Probability during Aging of Mice with Epidermal Deletion of DNA Methyltransferase 1 Ji Li,

Expression of Frizzled Genes in Developing and Postnatal Hair Follicles Seshamma T. Reddy, Thomas Andl, Min-Min Lu, Edward E. Morrisey, Sarah E. Millar,

Activated Kras Alters Epidermal Homeostasis of Mouse Skin, Resulting in Redundant Skin and Defective Hair Cycling Anandaroop Mukhopadhyay, Suguna R.

Gene Expression of Mouse S100A3, a Cysteine-Rich Calcium-Binding Protein, in Developing Hair Follicle Kenji Kizawa, Suguru Tsuchimoto, Keiko Hashimoto,

Expression of Frizzled Genes in Developing and Postnatal Hair Follicles Seshamma T. Reddy, Thomas Andl, Min-Min Lu, Edward E. Morrisey, Sarah E. Millar,

Regulation and Function of the Caspase-1 in an Inflammatory Microenvironment Dai-Jen Lee, Fei Du, Shih-Wei Chen, Manando Nakasaki, Isha Rana, Vincent.

CtBP1 Overexpression in Keratinocytes Perturbs Skin Homeostasis

The Transient Role for Calcium and Vitamin D during the Developmental Hair Follicle Cycle Leila J. Mady, Dare V. Ajibade, Connie Hsaio, Arnaud Teichert,

Adenovirus-Mediated Wnt10b Overexpression Induces Hair Follicle Regeneration Yu-Hong Li, Kun Zhang, Ke Yang, Ji-Xing Ye, Yi-Zhan Xing, Hai-Ying Guo, Fang.

Regulation of Hair Shedding by the Type 3 IP3 Receptor

Transglutaminase 5 Expression in Human Hair Follicle

Kai Kretzschmar, Denny L. Cottle, Pawel J. Schweiger, Fiona M. Watt

Conditional PDK1 Ablation Promotes Epidermal and T-Cell-Mediated Dysfunctions Leading to Inflammatory Skin Disease Minjun Yu, David M. Owens, Sankar.

Induction of Thymic Stromal Lymphopoietin Expression in Keratinocytes Is Necessary for Generating an Atopic Dermatitis upon Application of the Active.

Hair Cycle Resting Phase Is Regulated by Cyclic Epithelial FGF18 Signaling Miho Kimura-Ueki, Yuko Oda, Junko Oki, Akiko Komi-Kuramochi, Emi Honda, Masahiro.

James M. Waters, Jessica E. Lindo, Ruth M. Arkell, Allison J. Cowin

Wanglong Qiu, Xiaojun Li, Hongyan Tang, Alicia S. Huang, Andrey A

Desmoglein Isotype Expression in the Hair Follicle and its Cysts Correlates with Type of Keratinization and Degree of Differentiation Hong Wu Journal.

Vered Levy, Catherine Lindon, Brian D. Harfe, Bruce A. Morgan

Desmond J. Tobin Journal of Investigative Dermatology

Stem Cells with Neural Crest Characteristics Derived from the Bulge Region of Cultured Human Hair Follicles Hong Yu, Suresh M. Kumar, Andrew V. Kossenkov,

Atypical Protein Kinase C Isoform, aPKCλ, Is Essential for Maintaining Hair Follicle Stem Cell Quiescence Shin-Ichi Osada, Naoko Minematsu, Fumino Oda,

Ellen A. Rorke, Gautam Adhikary, Christina A. Young, Dennis R

Yasuyuki Amoh, Lingna Li, Kensei Katsuoka, Robert M. Hoffman

GW Bodies: Cytoplasmic Compartments in Normal Human Skin

Epidermal Label-Retaining Cells: Background and Recent Applications

Skin-Specific Deletion of Mis18α Impedes Proliferation and Stratification of Epidermal Keratinocytes Koog Chan Park, Minkyoung Lee, Yoon Jeon, Raok Jeon,

Peggy S. Myung, Makoto Takeo, Mayumi Ito, Radhika P. Atit

NF-κB Participates in Mouse Hair Cycle Control and Plays Distinct Roles in the Various Pelage Hair Follicle Types Karsten Krieger, Sarah E. Millar, Nadine.

Conditional Disruption of Hedgehog Signaling Pathway Defines its Critical Role in Hair Development and Regeneration Li Chun Wang, Zhong-Ying Liu, Renee.

Roles of GasderminA3 in Catagen–Telogen Transition During Hair Cycling

Fas and c-kit are Involved in the Control of Hair Follicle Melanocyte Apoptosis and Migration in Chemotherapy-Induced Hair Loss Andrei A. Sharov, Guang-Zhi.

Essential Role of the Keratinocyte-Specific Endonuclease DNase1L2 in the Removal of Nuclear DNA from Hair and Nails Heinz Fischer, Sandra Szabo, Jennifer.

Integrin β6-Deficient Mice Show Enhanced Keratinocyte Proliferation and Retarded Hair Follicle Regression after Depilation Yanshuang Xie, Kevin J. McElwee,

Localization of Plasminogen Activator Inhibitor Type 2 (PAI-2) in Hair and Nail: Implications for Terminal Differentiation Robert M. Lavker, Barbara.

Activation of Epidermal Growth Factor Receptor/ERK Signaling Correlates with Suppressed Differentiation in Malignant Acanthosis Nigricans Ingo Haase,

Transcription Factor CTIP2 Maintains Hair Follicle Stem Cell Pool and Contributes to Altered Expression of LHX2 and NFATC1 Shreya Bhattacharya, Heather.

Mohammad Rashel, Ninche Alston, Soosan Ghazizadeh

Fuz Controls the Morphogenesis and Differentiation of Hair Follicles through the Formation of Primary Cilia Daisy Dai, Huiping Zhu, Bogdan Wlodarczyk,

Mitochondrial Function in Murine Skin Epithelium Is Crucial for Hair Follicle Morphogenesis and Epithelial–Mesenchymal Interactions Jennifer E. Kloepper,

Lack of Collagen VI Promotes Wound-Induced Hair Growth

Hair Cycling and Wound Healing: To Pluck or Not to Pluck?

Slc1a3-CreER as a Targeting Tool for the K6+ Epithelial Stem Cell Niche and its Precursors during Mouse Hair Follicle Cycle Aiko Sada, Prachi Jain, Sherry.

Enpp2/Autotaxin in Dermal Papilla Precursors Is Dispensable for Hair Follicle Morphogenesis Laura Grisanti, Amelie Rezza, Carlos Clavel, Rachel Sennett,

Different Consequences of β1 Integrin Deletion in Neonatal and Adult Mouse Epidermis Reveal a Context-Dependent Role of Integrins in Regulating Proliferation,

The Cell Cycle Regulator Protein σ Is Essential for Hair Follicle Integrity and Epidermal Homeostasis Nigel L. Hammond, Denis J. Headon, Michael.

Yuko Oda, Lizhi Hu, Vadim Bul, Hashem Elalieh, Janardan K

Molecular Mechanisms Regulating Hair Follicle Development

Localization of Serine Racemase and Its Role in the Skin

Peptidylarginine Deiminase Isoforms Are Differentially Expressed in the Anagen Hair Follicles and Other Human Skin Appendages Rachida Nachat, Marie-Claire.

Transient Expression of Ephrin B2 in Perinatal Skin Is Required for Maintenance of Keratinocyte Homeostasis Gyohei Egawa, Masatake Osawa, Akiyoshi Uemura,

Chang-Yi Cui, Makoto Kunisada, Diana Esibizione, Eric G

Fate of Prominin-1 Expressing Dermal Papilla Cells during Homeostasis, Wound Healing and Wnt Activation Grace S. Kaushal, Emanuel Rognoni, Beate M. Lichtenberger,

Andreya Sharov, Desmond J. Tobin, Tatyana Y

Loss of γδ T Cells Results in Hair Cycling Defects

Delineating Immune-Mediated Mechanisms Underlying Hair Follicle Destruction in the Mouse Mutant Defolliculated Fiona Ruge, Aikaterini Glavini, Awen M.

Dihydrotestosterone-Inducible IL-6 Inhibits Elongation of Human Hair Shafts by Suppressing Matrix Cell Proliferation and Promotes Regression of Hair Follicles.

Multiple Epidermal Connexins are Expressed in Different Keratinocyte Subpopulations Including Connexin 31 Wei-Li Di, Elizabeth L. Rugg, Irene M. Leigh,

Thrombospondin-1 Plays a Critical Role in the Induction of Hair Follicle Involution and Vascular Regression During the Catagen Phase Kiichiro Yano, Michael.

Yap Controls Stem/Progenitor Cell Proliferation in the Mouse Postnatal Epidermis Annemiek Beverdam, Christina Claxton, Xiaomeng Zhang, Gregory James,

MHC Class I Expression in Murine Skin: Developmentally Controlled and Strikingly Restricted Intraepithelial Expression During Hair Follicle Morphogenesis.

Expression of Uncoupling Proteins in Human Skin and Skin-Derived Cells

Localized Inflammatory Skin Disease Following Inducible Ablation of I Kappa B Kinase 2 in Murine Epidermis Athanasios Stratis, Manolis Pasparakis, Doreen.

Yasushi Hanakawa, Hong Li, Chenyan Lin, John R

DLX3-Dependent STAT3 Signaling in Keratinocytes Regulates Skin Immune Homeostasis Shreya Bhattacharya, Jin-Chul Kim, Youichi Ogawa, Gaku Nakato, Veronica.

Y. Albert Pan, Joshua R. Sanes Journal of Investigative Dermatology

Claire A. Higgins, Gillian E. Westgate, Colin A.B. Jahoda

Tsutomu Soma, Cord E. Dohrmann, Toshihiko Hibino, Laurel A. Raftery

Presentation transcript:

Epidermal Stem Cells in the Isthmus/Infundibulum Influence Hair Shaft Differentiation: Evidence from Targeted DLX3 Deletion Jin-Chul Kim, Olivier Duverger, Joonsung Hwang, Maria I. Morasso Journal of Investigative Dermatology Volume 135, Issue 1, Pages 299-301 (January 2015) DOI: 10.1038/jid.2014.287 Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 DLX3 expression in the infundibulum/isthmus and cre recombinase activity in K14CreERT;R26RYFP. (a) Detection of DLX3 in the infundibulum/isthmus during telogen (P20) and first postnatal anagen (P24 and P26). DAPI (4,6-diamidino-2-phenylindole, blue) marks nuclei. Scale bar=50 μm. (b) Absence of DLX3 expression in sebaceous gland shown by immunohistochemistry with anti-DLX3 antibody. Scale bar=25 μm. (c) Detection of Lrig1 (red), DLX3 (green), and DAPI (blue) in the hair follicle at the telogen stage. Scale bar=25 μm. (d) Time schedule used for the analysis of cre recombinase activity in K14CreERT;R26RYFP mice. (e) Analysis of cre recombinase activity at telogen (P56) and 14 days after depilation (PD14). Yellow fluorescent protein (YFP) signal (green) marks cells in which cre recombination occurred. Immunohistochemical analysis of DLX3 (red) distribution was performed on the full anagen stage. Scale bar=25 μm. (f) YFP tracing and DLX3 staining on K14CreERT;R26RYFP (WT) and DLX3K14CreERT-cKO;R26RYFP (KO) skin at PD14. Scale bar=25 μm. Journal of Investigative Dermatology 2015 135, 299-301DOI: (10.1038/jid.2014.287) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Effects of DLX3 deletion in the infundibulum/isthmus on hair differentiation. (a) Time schedule of tamoxifen treatment and depilation applied on DLX3K14CreERT-cKO mice. Gross appearance of hair pelage (right side: depilated) and hematoxylin/eosin staining of skin sections (depilated). Scale bar=100 μm. (b) Immunohistochemical analysis of DLX3 (red) in depilated skin samples. DAPI (4,6-diamidino-2-phenylindole, blue) marks nuclei. Scale bar=50 μm. (c) Detection of type I hair keratins (AE13) and trichohyalin (AE15) in hair follicle bulbs from depilated skin at PD6 and PD14. K17 expression in medulla and outer root sheath from depilated skin at PD14. Scale bar=50 μm. (d) Scanning electron microscopy analysis of depilated skin from DLX3K14CreERT-cKO and control mice at PD14 showing hair structure. Lower panels show high-resolution images of the cuticle in control and DLX3K14CreERT-cKO mice. Scale bars: upper panels, 100 μm; lower panels, 25 μm. (e) Detection of lipid deposit in the wild-type (WT) and DLX3K14CreERT-cKO (KO) skin sections at telogen (P20). Red indicates Oil-Red-O staining. Scale bar=50 μm. Journal of Investigative Dermatology 2015 135, 299-301DOI: (10.1038/jid.2014.287) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions