K.P. Arkill, Ph.D., C.P. Winlove, D.Phil. Osteoarthritis and Cartilage

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Presentation transcript:

Solute transport in the deep and calcified zones of articular cartilage K.P. Arkill, Ph.D., C.P. Winlove, D.Phil. Osteoarthritis and Cartilage Volume 16, Issue 6, Pages 708-714 (June 2008) DOI: 10.1016/j.joca.2007.10.001 Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 A cross-section of demineralised metacarpal articular cartilage stained with toluidine blue. AC: articular cartilage, S: articular surface, T: tidemark, CC: calcified cartilage and SB: subchondral bone. Osteoarthritis and Cartilage 2008 16, 708-714DOI: (10.1016/j.joca.2007.10.001) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 A diagram of the diffusion chamber. Osteoarthritis and Cartilage 2008 16, 708-714DOI: (10.1016/j.joca.2007.10.001) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 A representative section of metacarpal articular cartilage from an intact joint perfused with rhodamine B base. The tidemarks are labelled (T). (A) Fluorescence image of rhodamine B base, showing the region from which an intensity profile was constructed. (B) Transmitted white light image of the same area. Osteoarthritis and Cartilage 2008 16, 708-714DOI: (10.1016/j.joca.2007.10.001) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 The equilibrium intensities (115h) of tracer in the calcified cartilage in diffusion chamber experiments. The intensity profiles for fluorescein (n=6) and rhodamine B base (n=15) and scaled by the concentration of tracer in the incubation medium as described in the text. Standard error bars are shown for each profile. Osteoarthritis and Cartilage 2008 16, 708-714DOI: (10.1016/j.joca.2007.10.001) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 The distribution of tracer in the calcified zone in diffusion chamber experiments for different incubation times. At each point the concentration is scaled by the equilibrium intensity, shown in Fig. 4, as described in the text. (A) Fluorescein (2h incubation: n=5, 5h: n=5 and 17h: n=6). (B) Rhodamine B base (2h: n=8, 5h: n=7, and 17h: n=5). Distance is measured from the tidemark and the approximate position of the cement line is indicated (C). Standard error bars are shown for each profile. Osteoarthritis and Cartilage 2008 16, 708-714DOI: (10.1016/j.joca.2007.10.001) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 Concentration profiles (relative to equilibrium concentration) after a 1.5h rhodamine B base perfusion of the intact joint. (A) Mean profiles aligned at the surface. Positive distance represents depth below the articular surface (n=26). (B) Mean profiles aligned at the tidemark (n=31). Standard error bars are shown. Osteoarthritis and Cartilage 2008 16, 708-714DOI: (10.1016/j.joca.2007.10.001) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 7 Concentration profiles after 1.5h perfusion of the intact joint Na-fluorescein and rhodamine B (0.1mg/ml). Profiles (scaled by equilibrium concentration) are aligned at the surface peak. Positive distance represents depth below the articular surface. (A) Unloaded joint (n=7). (B) Loaded joint (n=7). The ratio of rhodamine B (Rh) to Na-fluorescein (Na-Fl) is plotted on the second axis. Standard error bars are shown. Osteoarthritis and Cartilage 2008 16, 708-714DOI: (10.1016/j.joca.2007.10.001) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 8 Data from the experiment described in Fig. 7, but with concentration profiles aligned at the tidemark. (A) Unloaded joint (n=8). (B) Loaded joint (n=8). The ratio of rhodamine B (Rh) to Na-fluorescein (Na-Fl) is plotted on the second axis. Standard error bars are shown. Osteoarthritis and Cartilage 2008 16, 708-714DOI: (10.1016/j.joca.2007.10.001) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions