ABLE detects synchronously spiking, densely packed cells from mouse in vitro imaging data. ABLE detects synchronously spiking, densely packed cells from.

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ABLE detects synchronously spiking, densely packed cells from mouse in vitro imaging data. ABLE detects synchronously spiking, densely packed cells from mouse in vitro imaging data. The boundaries of the 207 detected ROIs are superimposed on the thresholded maximum intensity image (A) and the correlation image (D). For all correlation data we use Pearson’s correlation coefficient. ABLE detects ROIs that exhibit high correlation with the background (C) and neighboring synchronously spiking ROIs (B). B, Neuropil-corrected extracted time courses of the 207 ROIs (each plotted as a row of the matrix) along with the video mean raw activity and the time points of the electrical stimulations. C, Histogram of the correlation coefficient between the mean raw activity of the video and the extracted time series of each ROI. ABLE detected both active (E-G) and inactive ROIs (H, I). We display the contours of the two detected ROIs on the correlation image (E, H), the mean image (F, I) and the corresponding extracted time courses (G, J). Stephanie Reynolds et al. eNeuro 2017;4:ENEURO.0012-17.2017 ©2017 by Society for Neuroscience