Ephrin-A2 and Ephrin-A5 Are Important for the Functional Development of Cutaneous Innervation in a Mouse Model  Dulharie T. Wijeratne, Jennifer Rodger, Hilary J. Wallace, Siaavash Maghami, Matthew Sykes, Fiona M. Wood, Mark W. Fear  Journal of Investigative Dermatology  Volume 135, Issue 2, Pages 632-635 (February 2015) DOI: 10.1038/jid.2014.407 Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Interfollicular cutaneous innervation of Ephrin-A-/- and wild-type mice. (a–c) Interfollicular dermal nerve density in wild type, Ephrin-A2-/-, Ephrin-A5-/-, and Ephrin-A2A5-/- genotypes at P1 (a), P19 (b), and in adults (c). (d–f) Interfollicular epidermal nerve density in wild type, Ephrin-A2-/-, Ephrin-A5-/-, and Ephrin-A2A5-/- genotypes at P1 (d), P19 (e), and in adults (f). (g–r) Cutaneous epidermal and dermal nerves labeled with PGP 9.5 (marked with arrows) in wild type at P1 (g), Ephrin-A2-/- at P1(h), Ephrin-A5-/- at P1(i), Ephrin-A2A5-/- at P1 (j), wild type at P19 (k), Ephrin-A2-/- at P19 (l), Ephrin-A5-/- at P19 (m), Ephrin-A2A5-/- at P19 (n), adult wild type (o), adult Ephrin-A2-/- (p), adult Ephrin-A5-/- (q), and adult Ephrin-A2A5-/- (r). *P⩽0.05, **P⩽0.01, and ***P⩽0.001. Hair follicles are marked with arrowheads. Scale bar=50 μm. Journal of Investigative Dermatology 2015 135, 632-635DOI: (10.1038/jid.2014.407) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Interfollicular dermal and epidermal nerve density over time and sensory function in Ephrin A-/- and wild-type mice. (a–d) Interfollicular dermal nerve densities of wild type (a), Ephrin-A2-/- (b), Ephrin-A5-/- (c), Ephrin-A2A5-/- (d), genotypes across P1, P19, and adult stages. (e–h). Interfollicular epidermal nerve densities of wild type (e), Ephrin-A2-/- (f), Ephrin-A5-/- (g), Ephrin-A2A5-/- (h), genotypes across P1, P19, and adult stages. *P⩽0.05, **P⩽0.01, and ***P⩽0.001. (i) The touch sensitivity of Ephrin-A5-/- mice was significantly increased for 50% touch sensitivity threshold. *P<0.05 and **P<0.01. Ephrin-A5-/- mice were significantly less sensitive compared with wild-type (P=0.002), Ephrin-A2-/- (P=0.003), and Ephrin-A2A5-/- (P=0.024) mice. Journal of Investigative Dermatology 2015 135, 632-635DOI: (10.1038/jid.2014.407) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions