Implanted pedicled autologous pericardium mimics vasculature tissue: Case report  Naoki Masaki, MD, Osamu Adachi, MD, PhD, Shunsuke Kawamoto, MD, PhD,

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Implanted pedicled autologous pericardium mimics vasculature tissue: Case report  Naoki Masaki, MD, Osamu Adachi, MD, PhD, Shunsuke Kawamoto, MD, PhD, Yoshikatsu Saiki, MD, PhD  The Journal of Thoracic and Cardiovascular Surgery  Volume 153, Issue 1, Pages e11-e13 (January 2017) DOI: 10.1016/j.jtcvs.2016.09.027 Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 Angiography of the right ventricular outflow tract showing enlargement of the outflow tract from the early postoperative time (A) to 14 years after the operation (B). The Journal of Thoracic and Cardiovascular Surgery 2017 153, e11-e13DOI: (10.1016/j.jtcvs.2016.09.027) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 The histology of pedicled autologous pericardium tissue used for right ventricular outflow tract reconstruction. A, Hematoxylin and eosin staining. B, Elastica-Masson staining showing a layer of elastic tissue with a layer of smooth muscle cells beneath the elastic layer. C, Staining for α-smooth muscle actin showing positive cells, confirming the presence of smooth muscle cells. D, Staining for CD31 revealing abundant microvasculature in the pedicled autologous pericardium tissue. The Journal of Thoracic and Cardiovascular Surgery 2017 153, e11-e13DOI: (10.1016/j.jtcvs.2016.09.027) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Abundant microvasculature and emersion of smooth muscle cells in the pedicled pericardium. The Journal of Thoracic and Cardiovascular Surgery 2017 153, e11-e13DOI: (10.1016/j.jtcvs.2016.09.027) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions