Activation of miR‐21‐3p by PPARβ/δ requires activation of the TGFβ receptor Activation of miR‐21‐3p by PPARβ/δ requires activation of the TGFβ receptor.

Slides:

Advertisements

Similar presentations

The expressions of H19 and Igf1r are significantly decreased in the eutopic endometrium of women with endometriosis compared to those without endometriosis.

Advertisements

Hyp mice show hypertension and increased NCC expression and phosphorylation A–ESerum intact Fgf23 concentration (A, n = 8–9, Student's t‐test, *P =

Effects of inhibiting inflammatory cell recruitment on fracture healing Treatment with CCR2 antagonist, INCB3344, led to impaired fracture healing compared.

Loss of Hdac3 impairs Ar signaling in mouse prostate tissues and has no effect on apoptosis Loss of Hdac3 impairs Ar signaling in mouse prostate tissues.

Reduced astrocyte proliferation results in increased immune cell infiltration into the injured GM Reduced astrocyte proliferation results in increased.

Metabolic effects of TUG‐891 are reduced or absent in GPR120‐deficient mice Metabolic effects of TUG‐891 are reduced or absent in GPR120‐deficient mice.

HDAC3 regulates AKT phosphorylation

Trx80 is generated by α‐secretases. A

The HDAC3 inhibitor suppresses SPOP‐mutated prostate cancer cell growth The HDAC3 inhibitor suppresses SPOP‐mutated prostate cancer cell growth A22Rv1.

TUG‐891 increases oxygen consumption by brown adipocytes, mediated by direct UCP1 activation and mitochondrial fragmentation TUG‐891 increases oxygen consumption.

PbA‐specific CD4 T‐cell responses are maximal at day 6 post‐pRBC infection PbA‐specific CD4 T‐cell responses are maximal at day 6 post‐pRBC infection Proportion.

IFNα, but not IFNλ, treatment induces cytokine secretion from human immune cells IFNα, but not IFNλ, treatment induces cytokine secretion from human immune.

Treatment with p38γ/p38δ inhibitor shows antifungal effects in vivo

[68Ga]Pentixafor PET imaging of MM xenografts Real‐time PCR analysis of cxcr4 transcript expression levels in HeLa (negative control) and in MM cell lines.

Skin proliferation kinetics and in vivo fibroblast lineage tracing during dermal maturation (related to Fig 4)‏ Skin proliferation kinetics and in vivo.

AAV8‐hAAT‐FGF21 treatment improves liver fibrosis

Metabolic profile of Tfe3 KO mice

Effect of PRK and SRB on Mtb H37Rv survival

Triple‐negative breast cancer (TNBC) cells in human express a specific gene signature and their self‐renewal depends on canonical Wnt/β‐catenin signalling.A.Human.

Characterization of promoters relative to pAGA1

Clinical testing of calcineurin inhibitors cyclosporine A and tacrolimus in autoimmune disorders Clinical testing of calcineurin inhibitors cyclosporine.

Stat3 promotes lung fibrosis and collagen synthesis independent of Smad3.A.Masson's trichrome stained sections of lung from gp130wt (wt), Smad3−/− (Smad3−/−),

Inhibition of JNK signaling in breast cancer cells and lung colonization in a xenograft mouse model Inhibition of JNK signaling in breast cancer cells.

KLK7 is involved in the Aβ degradation activity

Expression of PD‐1 on PBMC and malignant exudate T cells

Identification of miR‐499 targets

The BET inhibitor JQ‐1 in combination with the MEK inhibitor PD synergistically impairs cell proliferation and induces apoptosis of NRAS‐mutant.

TR specifically inhibits NLRP3 inflammasome activation

Combination of OTX‐015 and PD does not induce overt toxicity

Stat3C/C mice are more prone to Th17 differentiation. A

Dietary administration of DHEA reduces jet‐lag

Zc3h10 overexpression boosts mitochondrial function and density in myotubes Zc3h10 overexpression boosts mitochondrial function and density in myotubes.

Zc3h10 downregulation impairs mitochondrial function and C2C12 differentiation Zc3h10 downregulation impairs mitochondrial function and C2C12 differentiation.

Effect of Zc3h10 overexpression in myoblasts and myotubes

BETi potentiate the efficacy of MEKi in NRAS‐mutant melanoma

The PPAR-α agonist GW7647 increases the levels of apoA-I in the retina and the fibrous sclera. The PPAR-α agonist GW7647 increases the levels of apoA-I.

The PPAR-α agonist GW7647 reduces experimentally induced myopia.

Effects of in vivo Nutlin‐3 treatment on CSC content of Numb− and Numb+ tumors Effects of in vivo Nutlin‐3 treatment on CSC content of Numb− and Numb+

The ERK pathway mediates the effect of EGF on LIMT, which normally inhibits mammary cell migration and invasion The ERK pathway mediates the effect of.

TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells A, BWestern.

The preventive role of TR in HFD‐treated mice

IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice ARepresentative.

LV.IDS and LV.IDS.ApoEII improve brain‐specific IDS activity and express supra‐physiological levels of active IDS in peripheral organs LV.IDS and LV.IDS.ApoEII.

Inhibition of NAMPT using the potent inhibitor FK866 sensitizes additional tumour cells to olaparib.A‐E.Sensitivity of the cell lines MDAMB468 (A), SUM149.

Impact of ONX 0914 on abundance and dissemination of monocytes/macrophages during CVB3 infection Impact of ONX 0914 on abundance and dissemination of monocytes/macrophages.

Characterization of MET‐addicted cells rendered resistant to MET tyrosine kinase inhibitors Characterization of MET‐addicted cells rendered resistant to.

Surface biotinylation and fate of TREM2

Gain of FGF23 function induces renal Na+ retention through increased renal NCC expression and channel activation AUrine volume (n = 15–17), urinary Na+

GPR120 deficiency alters the expression of genes involved in glucose and lipid metabolism in BAT GPR120 deficiency alters the expression of genes involved.

Target engagement of the systemic Stat3 inhibitor in APP/PS1 mice

Lack of RAD51 nuclear foci identifies PARPi‐sensitive PDX tumors

The HDAC3 inhibitor suppresses PTEN‐deficient prostate cancer growth

Effect of memantine on Klk7‐dependent Aβ degradation activity

I.c.v.‐injected AβOs induce increased food intake, hypothalamic expression of orexigenic neuropeptides but no hypothalamic cell degeneration AAccumulated.

The antitumor activity of olaparib in PDXs identifies a subset of PARPi‐sensitive tumors The antitumor activity of olaparib in PDXs identifies a subset.

Therapeutic role of TR in HFD‐established diabetic mice

DHEA shortens circadian period

P2X4R blockade increases autoimmune inflammation

Treatment with AZD5363 reduces the proportion of proliferating vascular endothelial cells and following RT, the influx of CD11b+ bone marrow‐derived cells.

Etifoxine modulation of T‐cell activity during EAE

Histological and mRNA analysis of the inflammatory cytokines in the vehicle‐ or etifoxine‐treated mice at onset of clinical symptoms.A–D.At day 10 p.i.,

The MEK inhibitor CI‐1040 reverses thickening, fibrosis, and angiogenesis in the parietal peritoneum of Cav1−/− mice upon exposure to PD fluidWT or Cav1−/−

Tissue‐specific differences in the levels of CoQ9, CoQ10, and DMQ9, and in the DMQ9/CoQ9 ratio after β‐RA treatment in Coq9R239X mice Tissue‐specific differences.

P2X4R blockade increases pro‐inflammatory gene expression after EAE

Nilotinib inhibits DDR1 invasive activity of patient‐derived CRC cells

CNP520 treatment of male C57/BL6 mice for 8 weeks did not cause hair depigmentation CNP520 treatment of male C57/BL6 mice for 8 weeks did not cause hair.

Limiting neutrophilic inflammation during active phase (ZT13) reduces MI damage Limiting neutrophilic inflammation during active phase (ZT13) reduces MI.

Absence of miR‐21 in macrophages promotes foam cell formation

KlbHET mice exhibit altered estrous cycle and subfertility

Niacin suppresses pro‐inflammatory cytokine expression in macrophages in DSS‐induced colitis in mice Niacin suppresses pro‐inflammatory cytokine expression.

Presentation transcript:

Activation of miR‐21‐3p by PPARβ/δ requires activation of the TGFβ receptor Activation of miR‐21‐3p by PPARβ/δ requires activation of the TGFβ receptor RT–qPCR quantification of relative pri‐miR‐21 level in HaCaT human keratinocytes treated with the PPARβ/δ agonist GW0742 (+) or vehicle (−), with (+) or without (−) cycloheximide (Cyclo) as indicated. Pri‐miR‐21: Veh vs. GW0742 P = 0.009. N = 3 biological replicates, one representative experiment is shown out of two independent replicates.RT–qPCR quantification of relative pri‐miR‐21, pre‐miR‐21, miR‐21‐5p, and miR‐21‐3p levels in HaCaT cells treated for 24 h with 2 or 5 ng/ml of recombinant human TGFβ1 (+) or vehicle (−) as indicated. Pri‐miR‐21: Veh vs. TGFβ1 5 ng/ml P = 0.029; Pre‐miR‐21: Veh vs. TGFβ1 5 ng/ml P = 0.001; miR‐21‐5p: Veh vs. TGFβ1 5 ng/ml P = 0.002; miR‐21‐3p: Veh vs. TGFβ1 5 ng/ml P = 1.7E‐05. N = 3 biological replicates, one representative experiment is shown out of two independent replicates.RT–qPCR quantification of pri‐miR‐21, pre‐miR‐21, miR‐21‐5p, and miR‐21‐3p levels in HaCat cells treated for 24 h with the PPARβ/δ agonist GW0742 (+), TGFβ receptor inhibitor SB431542 (+), or vehicle (−) as indicated. Pri‐miR‐21: GW0742 vs. SB431542 P = 0.004, GW0742 vs. GW0742/SB431542 P = 0.015; Pre‐miR‐21: Veh vs. GW0742 P = 0.022, GW0742 vs. SB431542 P = 0.036, GW0742 vs. GW0742/SB431542 P = 0.024; miR‐21‐5p: GW0742 vs. SB431542 P = 0.034, GW0742 vs. GW0742/SB431542 P = 0.024; miR‐21‐3p: Veh vs. GW0742 P = 0.036, GW0742 vs. SB431542 P = 0.011, GW0742 vs. GW0742/SB431542 P = 0.008. N = 3 biological replicates, one representative experiment is shown out of two independent replicates.RT–qPCR quantification of relative Tgfb1 level in the epidermis of acutely irradiated (Ac‐UV; +) and non‐irradiated (−) Ppard+/+ and Ppard−/− mice. Tfgb1: Ppard+/+ no UV vs. Ac‐UV P = 0.034, Ppard+/+ Ac‐UV vs. Ppard−/− Ac‐UV P = 0.030. N = 2–3 animals per group, one representative experiment is shown out of three independent replicates.RT–qPCR quantification of relative miR‐21‐5p and miR‐21‐3p levels in the skin of Ppard+/+ mice treated with the TGFβ receptor inhibitor SB431542 (+) or vehicle (−), with (+) or without (−) acute UV exposure (Ac‐UV). miR‐21‐5p: no UV vs. Ac‐UV P = 0.038, Ac‐UV vs. Ac‐UV/SB431542 P = 0.028; miR‐21‐3p: no UV vs. Ac‐UV P = 0.017; Ac‐UV vs. Ac‐UV/SB431542 P = 0.035. N = 3 animals per group, one representative experiment is shown out of three independent replicates.Data information: Results are presented as mean values ± SEM. The statistical comparison between groups was performed by using t‐test. *P‐value < 0.05; **P‐value < 0.01. Gwendoline Degueurce et al. EMBO Mol Med. 2016;emmm.201505384 © as stated in the article, figure or figure legend