Semen analysis by electron and fluorescence microscopy in a case of partial hydatidiform mole reveals a high incidence of abnormal morphology, diploidy,

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Presentation transcript:

Semen analysis by electron and fluorescence microscopy in a case of partial hydatidiform mole reveals a high incidence of abnormal morphology, diploidy, and tetraploidy Katerina Chatzimeletiou, M.Sc., Ph.D., Antonia Sioga, Ph.D., Louisa Oikonomou, M.D., Ph.D., Sophia Charalampidou, M.D., Persa Kantartzi, M.D., Vasiliki Zournatzi, M.D., Ph.D., Dimitrios Panidis, M.D., Ph.D., Dimitrios G. Goulis, M.D., Ph.D., Ioannis Papadimas, M.D., Ph.D., Basil C. Tarlatzis, M.D., Ph.D. Fertility and Sterility Volume 95, Issue 7, Pages 2430.e1-2430.e5 (June 2011) DOI: 10.1016/j.fertnstert.2011.01.161 Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 1 TEM photomicrographs showing sperm with (A) abnormalities in the acrosome and the midpiece, (B) higher magnification of the sperm in (A), and (C) abnormal acrosome (arrow). Also note the normal tails around the sperm head (arrow heads), (D) abnormal tail and cytoplasmic remnants, (E) immature spermatid, and (F–I) abnormal spermiogenesis. Note that only part of the tail is created in H (arrow), while only the head and acrosome is created in I. (J) Sperm that has been phagocytosed by a macrophage. Fertility and Sterility 2011 95, 2430.e1-2430.e5DOI: (10.1016/j.fertnstert.2011.01.161) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 2 FISH photomicrographs showing sperm hybridized with the (A–C) Multivysion PB probe (chromosomes 13-red, 16-aqua, 18-blue, 21-green, 22-gold) and the (D–I) Aneuvysion probe (chromosomes X-green, Y-red, 18-aqua). (A) Normal haploid sperm, (B) aneuploid sperm showing no signal for chromosome 13 (red) and two signals for chromosome 21 (green), (C) diploid sperm showing two signals for all five chromosomes tested, (D) normal sperm (X, 18), (E) normal sperm (Y, 18), (F) diploid sperm (XY1818), (G) diploid sperm (XX1818), (H) diploid sperm (YY1818), and (I) tetraploid sperm (XXYY18181818). Fertility and Sterility 2011 95, 2430.e1-2430.e5DOI: (10.1016/j.fertnstert.2011.01.161) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions