Expression of two-pore domain potassium channels in nonhuman primate sperm  Gregory E. Chow, M.D., Charles H. Muller, Ph.D., Eliza C. Curnow, M.Sc., Eric S. Hayes, Ph.D.  Fertility and Sterility  Volume 87, Issue 2, Pages 397-404 (February 2007) DOI: 10.1016/j.fertnstert.2006.06.051 Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 Demonstrates the expression of (A) TRAAK, (B) TREK-1, and (C) TASK-2 in nonhuman primate sperm (Macaca nemestrina). Column 1, rat brain extract; column 2, native sperm. *Protein bands at approximately 100 kd. Chow. Potassium ion channels in monkey sperm. Fertil Steril 2007. Fertility and Sterility 2007 87, 397-404DOI: (10.1016/j.fertnstert.2006.06.051) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Illustrates the regional distributions of (A, a) TRAAK, (B, b) TREK-1, and (C, c) TASK-2 in nonhuman primate sperm (M. nemestrina). Sperm exposed to secondary antibody in the absence of exposure to primary antibodies directed against K2P channel isoforms served as negative controls (D, d). Images with capital letters are light microscope images, whereas images with lower case letters represent fluorescent images for each K2P isoform. The horizontal bar indicates a distance of 10 μm. Chow. Potassium ion channels in monkey sperm. Fertil Steril 2007. Fertility and Sterility 2007 87, 397-404DOI: (10.1016/j.fertnstert.2006.06.051) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Illustrates the regional distributions of (A, a) TRAAK, (B, b) TREK-1, and (C, c) TASK-2 in nonhuman primate sperm (M. nemestrina). Sperm exposed to secondary antibody in the absence of exposure to primary antibodies directed against K2P channel isoforms served as negative controls (D, d). Images with capital letters are light microscope images, whereas images with lower case letters represent fluorescent images for each K2P isoform. The horizontal bar indicates a distance of 10 μm. Chow. Potassium ion channels in monkey sperm. Fertil Steril 2007. Fertility and Sterility 2007 87, 397-404DOI: (10.1016/j.fertnstert.2006.06.051) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions