The Small RNA Profile during Drosophila melanogaster Development

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The Small RNA Profile during Drosophila melanogaster Development Alexei A. Aravin, Mariana Lagos-Quintana, Abdullah Yalcin, Mihaela Zavolan, Debora Marks, Ben Snyder, Terry Gaasterland, Jutta Meyer, Thomas Tuschl Developmental Cell Volume 5, Issue 2, Pages 337-350 (August 2003) DOI: 10.1016/S1534-5807(03)00228-4 Copyright © 2003 Cell Press Terms and Conditions

Figure 1 miRNA Genes mir-100, let-7 and the lin-4 Homolog mir-125 Are Clustered (A) Arrangement of the miRNA genes in D. melanogaster. The 70 nt fold-back precursor is indicated as box, the position of the miRNA in the precursor is shown in black. The chromosome location is indicated to the right. (B) Northern blots confirming the coexpression of miR-100, let-7, and miR-125 in pupa and adult. As control for loading the 30 nt 2S rRNA band was visualized by ethidium bromide staining of the polyacrylamide gel before transfer of the RNA to the blotting membrane. Development stages of embryos are indicated in hours after egg laying. Larval stages are indicated as L1, L2, and L3. P indicates pupal stage; M, adult male; F, adult female. (C) The miRNA clustering is conserved between invertebrates and vertebrates but gene duplication occurred in mammals and the spacing between miRNA precursors increased with increasing genome size. Developmental Cell 2003 5, 337-350DOI: (10.1016/S1534-5807(03)00228-4) Copyright © 2003 Cell Press Terms and Conditions

Figure 2 Expression Profiles of a Subset of Identified miRNA Genes Northern blotting was performed as described in the legend to Figure 1B. Developmental Cell 2003 5, 337-350DOI: (10.1016/S1534-5807(03)00228-4) Copyright © 2003 Cell Press Terms and Conditions