UVB Irradiation Stimulates Deposition of New Elastic Fibers by Modified Epithelial Cells Surrounding the Hair Follicles and Sebaceous Glands in Mice

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UVB Irradiation Stimulates Deposition of New Elastic Fibers by Modified Epithelial Cells Surrounding the Hair Follicles and Sebaceous Glands in Mice Barry Starcher Journal of Investigative Dermatology Volume 112, Issue 4, Pages 450-455 (April 1999) DOI: 10.1046/j.1523-1747.1999.00553.x Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 In situ hybridization for tropoelastin and collagen α1(I). Message levels for tropoelastin are shown in control mouse skin (a) and in mouse skin after 5 d of UVB (b), 8 wk of UVB (c), and 20 wk of UVB (d). Collagen message levels are shown for control mouse skin (i) and in mouse skin after 5 d of UVR (j), 8 wk of UVB (k), and 20 wk of UVB (l). Hematoxylin and eosin-stained light field photos illustrating the concentration of inflammatory cells are shown in (e)–(h). Scale bar: 0.5 μm. Journal of Investigative Dermatology 1999 112, 450-455DOI: (10.1046/j.1523-1747.1999.00553.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Mouse skin sections stained for elastin. Longitudinal section of 25 wk UVB-irradiated mouse skin (a) and normal control skin (b). (c) En face section of control skin just under the dermal–epidermal junction. En face section of elastic fiber band encircling (e) the follicular cysts and (f) the subpannicular elastin fibers in normal control skin. En face section just below the dermal–epidermal junction of UV-irradiated skin (d) illustrating the orientation and increase in elastin fibers. (g) En face section of elastin fibers emanating from the cells surrounding the sebaceous gland. (h) Elongated and straightened appearance of subpanniculus elastin fibers sectioned en face following UV irradiation. (i) Immunostaining for tropoelastin (blue) surrounding an epithelial root sheath sectioned en face. White scale bars: 25 μm;black scale bars: 50 μm. Journal of Investigative Dermatology 1999 112, 450-455DOI: (10.1046/j.1523-1747.1999.00553.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Electron micrographs showing (a) the area directly adjacent to a sebaceous gland and (b) immunogold localization with anti-elastin antibody. (a) The sebaceous gland is just visable on the left bottom. The gland is lined with flattened epithelial cells typical of stratum corneum (EP) containing modified desmosomes. Above this area are cells containing elastin deposits stained dark with tannic acid (E). The junctional surface of the cells show numerous desmosomes (arrows). Scale bar: 0.5 μm. (b) Immunogold grains specifically localized within the intracellular elastin deposits (E) of modified epithelial cells surrounding a sebaceous gland (SG). Scale bar: 2 μm. Journal of Investigative Dermatology 1999 112, 450-455DOI: (10.1046/j.1523-1747.1999.00553.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Desmosine analysis of biopsy punches sectioned en face. En face sectioning from the epidermis in 100 μm increments through the skin and including the elastin band lying beneath the panniculus carnosus muscle. Control skin shown in solid circles (•) and UVB-irradiated skin in open circles (○). Journal of Investigative Dermatology 1999 112, 450-455DOI: (10.1046/j.1523-1747.1999.00553.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Desmosine and hydroxyproline analysis of 3 mm biopsy punch of mouse skin. Desmosine is shown in solid bars and collagen in hatched bars. Each value is the average of 10 samples with 1 SD. Journal of Investigative Dermatology 1999 112, 450-455DOI: (10.1046/j.1523-1747.1999.00553.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions